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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant human Creatine kinase M-type protein
- 形态:
liquid
- 保存条件:
Store at -20˚C
- 克隆性:
Polyclonal
- 适应物种:
Human
- 保质期:
12 months
- 抗原来源:
Rabbit
- 供应商:
南京赛戈巍生物科技有限公司
- 宿主:
Rabbit
- 应用范围:
WB, IF
- 靶点:
Uniprot:P06732
- 抗体英文名:
Creatine kinase M-type Polyclonal Conjugated Antibody
- 规格:
50ul/100ul
计算分子量:42
配方:0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 5mg/ml Bovine Serum Albumin, 0.02% Sodium Azide
应用详情:WB: 1:50-1:200
IF:1:50-1:200
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文献和实验Polyclonal and Monoclonal Antibodies
Polyclonal Antibody Immunization of Mice: Injections should be made at intervals of at least two weeks.. We have been using either of the two following adjuvants with success: Freund adjuvant and MPL+TDM adjuvant ( RIBI ImmunoChem
C), Roche # 11 017 101 001 Aprotinin 10 mg/mL in PBS (5000-160,000x, aliquot and store -20°C), Roche # 10 981 532 001 PMSF: (phenylmethylsulfonyl flouride, 100x) 0.2 M in ethanol. Store at 4°C. 100x Phosphatase inhibitor mix (store -20ºC
Identification of a Mutant Kinase/ATP Analog Pair
available phospho-specific antibody to the ERK2 substrate Elk1. A phospho-specific antibody allows a large number of ATP analogs to be screened without their having to be made radioactive. In this assay, active wild-type or mutant kinase is mixed with ATP
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