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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant human Poly(A)-specific ribonuclease PARN protein
- 形态:
liquid
- 保存条件:
Store at -20˚C
- 克隆性:
Polyclonal
- 适应物种:
Human
- 保质期:
12 months
- 抗原来源:
Rabbit
- 供应商:
南京赛戈巍生物科技有限公司
- 宿主:
Rabbit
- 应用范围:
WB, IF
- 靶点:
Uniprot:O95453
- 抗体英文名:
Poly(A)-specific ribonuclease PARN Polyclonal Conjugated Antibody
- 规格:
50ul/100ul
计算分子量:73
配方:0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 5mg/ml Bovine Serum Albumin, 0.02% Sodium Azide
应用详情:WB: 1:50-1:200
IF:1:50-1:200
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文献和实验T-cell dependent antigen specific in situ staining (Pe)
-conjugated secondary antibody (Typically SBA goat anti rabbit-AP 1:100, rabbit anti goat-AP 1:300) 22- wash twice 23- [optional] add a tertiary layer (rabbit anti-goat-AP on a goat anti-rabbit-AP) 24- wash thrice 25- develop AP (see protocol
In situ hybridisation to alpha satellite sequences (chromosome specific)
Alpha satellite sequences, whilst highly repetitive, are specific to each individual chromosome. These sequences flank the centromeres and can present a target measured in megabases. In this protocol a biotin or digoxigenin labelled DNA
case, the antibody should be added in 1:100 to 1:500 final dilution. • For Hsp70, 3A3 and C92 did work, 4G4 and 5A5 did not. 3A3 may be used 1 μl/ sample. • HSE probe must have high-specific activity (>10,000 cpm/fmol) and be double-stranded
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