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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant human Proteasome subunit beta type-4 protein
- 形态:
liquid
- 保存条件:
Store at -20˚C
- 克隆性:
Polyclonal
- 适应物种:
Human
- 保质期:
12 months
- 抗原来源:
Rabbit
- 供应商:
南京赛戈巍生物科技有限公司
- 宿主:
Rabbit
- 应用范围:
WB, IF
- 靶点:
Uniprot:P28070
- 抗体英文名:
Proteasome subunit beta type-4 Polyclonal Conjugated Antibody
- 规格:
50ul/100ul
计算分子量:29
配方:0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 5mg/ml Bovine Serum Albumin, 0.02% Sodium Azide
应用详情:WB: 1:50-1:200
IF:1:50-1:200
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文献和实验Studies of the Ubiquitin Proteasome System
antibody for the protein of interest and isotype‐matched control antibody, e.g., irrelevant or preimmune serum, purified Tris‐buffered saline/Tween 20 (TBST; see recipe ), 4°C Cells growing in culture
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
secondary antibody. For double-labeling experiments, use fluorescence-conjugated secondary antibodies raised to the corresponding type of primary antibody. The dilution factor for each antibody needs to be determined experimentally. Blocking
Large-scale immunofluorescence
overnight7 at 4o C with very gentle shaking. Wash cells once with PBS, once with PBS plus 0.1% NP40, and then again with PBS. Resuspend cells in 30 ul of PBS plus 0.1% BSA containing the secondary antibody8 , and incubate for 4 hours at room
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