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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
A synthetic methylated peptide corresponding to residues surrounding R8 of human histone H3
- 形态:
liquid
- 保存条件:
Store at -20˚C
- 克隆性:
Polyclonal
- 适应物种:
Hu Ms Rt Other (Wide Rtange)
- 保质期:
12 months
- 抗原来源:
Rabbit
- 供应商:
南京赛戈巍生物科技有限公司
- 宿主:
Rabbit
- 应用范围:
WB, IF
- 靶点:
Uniprot:Q16695
- 抗体英文名:
Histone H3R8me2aConjugated Antibody
- 规格:
50ul/100ul
计算分子量:15
配方:0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 5mg/ml Bovine Serum Albumin, 0.02% Sodium Azide
应用详情:WB: 1:50-1:200
IF:1:50-1:200
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文献和实验9.19 Nature:揭示组蛋白标记 H3K36me2 招募 DNMT3A 并影响基因间 DNA 甲基化
中心、洛克菲勒大学和加拿大麦吉尔大学等研究机构的研究人员报道 NSD1 介导的 H3K36me2 是在基因间区域招募 DNMT3A 和维持 DNA 甲基化所必需的。这项新的研究揭示了一种跨染色质调节途径,这种调节突进将异常的基因间 CpG 甲基化与人类肿瘤生长和过度发育相关联在一起。原文检索:The histone mark H3K36me2 recruits DNMT3A and shapes the intergenic DNA methylation landscape ② Nature 突破
PROTOCOL To 1.5 mL eppendorf tubes add: 200 µg of protein extract (see Western blot protocol for protein sample preps) q.s. to 300 µL with RIPA (with protease and phosphatase inhibitors). Primary antibody (amount determined
Chromatin Immunoprecipitation Protocol for Histone Modification
. This has been very successful when using antibodies that detect site specific histone lysine methylation (Peters et al . 2003, Martens et al . 2005). However, for each different antibody the optimal concentration should be tested and calibrated. The stringency of washing
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