- 询价
- Signalway Antibody
- C49973
- 2025年09月23日
- WB, IF
- Rabbit
- Hu, Ms, Rt
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein corresponding to C-terminal human Lysyl tRNA synthetase.
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Monoclonal
- 适应物种：
Hu, Ms, Rt
- 保质期：
12 months
- 抗原来源：
Rabbit
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
Rabbit
- 应用范围：
WB, IF
- 靶点：
Uniprot：Q15046
- 抗体英文名：
Lysyl tRNA synthetase Conjugated Antibody
- 规格：
50ul/100ul
DFNB89 antibody
EC 6.1.1.6 antibody
KARS 1 antibody
KARS 2 antibody
KARS antibody
KARS1 antibody
KARS2 antibody
KIAA0070 antibody
KRS antibody
Lysine tRNA ligase antibody
Lysine--tRNA ligase antibody
LysRS antibody
Lysyl-tRNA synthetase antibody
SYK_HUMAN antibody
计算分子量:68 kDa
配方:0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 5mg/ml Bovine Serum Albumin, 0.02% Sodium Azide
应用详情:WB: 1:50-1:200
IF:1:50-1:200
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文献和实验Non-fluorescent protein/RNA double-labeling
, 50 ug/ml heparin, and 250 ug/ml tRNA). You can also include 1% BSA providing you know it is ultra pure/RNase-free. With our reagents, we find we get similar results without it. 4. Incubate with the primary antibody (diluted appropriately in PBTH
Non-fluorescent protein/RNA double-labeling
minutes each: (i) hybridization buffer alone (ii) PBT:hybridization buffer (1:1) (iii) PBT alone for at least 5 washes 11. Incubate with anti-Digoxygenin antibody (Boerhinger Mannheim) conjugated to alkaline phosphatase (diluted 1:2000
Sequential RNA and DNA fluorescence in situ hybridization
with 1XSSC 3 times for 5 minutes each at 60 °C; Incubate with 1% H2 O2 in PBS for 30 minutes at RT (comment 1); Block with 3% BSA/ 2XSSC/ 0.1% Tween 20 for 15 minutes at RT; Incubate with a fluorophore-conjugated anti-DIG antibody diluted
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