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- 详细信息
- 文献和实验
- 技术资料
- 形态:
liquid
- 保存条件:
Store at -20˚C
- 克隆性:
Monoclonal
- 适应物种:
Hu, Rt
- 保质期:
12 months
- 抗原来源:
Rabbit
- 供应商:
南京赛戈巍生物科技有限公司
- 宿主:
Rabbit
- 应用范围:
WB, IF
- 靶点:
Uniprot:P18858
- 抗体英文名:
DNA Ligase I Conjugated Antibody
- 规格:
50ul/100ul
DNA ligase I antibody
DNLI1_HUMAN antibody
LIG 1 antibody
lig1 antibody
Ligase I DNA ATP dependent antibody
MGC117397 antibody
MGC130025 antibody
Polydeoxyribonucleotide synthase [ATP] 1 antibody
计算分子量:Predicted band size 102 kDa
配方:0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 5mg/ml Bovine Serum Albumin, 0.02% Sodium Azide
应用详情:WB: 1:50-1:200
IF:1:50-1:200
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文献和实验Sequential RNA and DNA fluorescence in situ hybridization
with 1XSSC 3 times for 5 minutes each at 60 °C; Incubate with 1% H2 O2 in PBS for 30 minutes at RT (comment 1); Block with 3% BSA/ 2XSSC/ 0.1% Tween 20 for 15 minutes at RT; Incubate with a fluorophore-conjugated anti-DIG antibody diluted
Sequential RNA and DNA fluorescence in situ hybridization
with 1XSSC 3 times for 5 minutes each at 60 °C; Incubate with 1% H2 O2 in PBS for 30 minutes at RT (comment 1); Block with 3% BSA/ 2XSSC/ 0.1% Tween 20 for 15 minutes at RT; Incubate with a fluorophore-conjugated anti-DIG antibody diluted
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
7 mL of 1.15X ligation buffer. 19. Add 400 µL of 20% Triton X-100 (to a final concentration of 1%). Incubate with occasional gentle shaking for 1 h at 37°C. 20. Add 50 µL of T4 DNA ligase. Incubate for 4 h at 16°C
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