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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
recombinant protein
- 形态:
liquid
- 保存条件:
Store at -20˚C
- 克隆性:
Monoclonal
- 适应物种:
Hu, Rt
- 保质期:
12 months
- 抗原来源:
Rabbit
- 供应商:
南京赛戈巍生物科技有限公司
- 宿主:
Rabbit
- 应用范围:
WB, IF
- 靶点:
Uniprot:P35749
- 抗体英文名:
smooth muscle Myosin heavy chain 11 Conjugated Antibody
- 规格:
50ul/100ul
DKFZp686D10126 antibody
DKFZp686D19237 antibody
FAA4 antibody
FLJ35232 antibody
MGC126726 antibody
MGC32963 antibody
MYH 11 antibody
MYH11 antibody
MYH11_HUMAN antibody
Myosin 11 antibody
Myosin heavy chain 11 antibody
Myosin heavy chain 11 smooth muscle antibody
Myosin heavy chain antibody
Myosin heavy chain smooth muscle isoform antibody
Myosin heavy polypeptide 11 smooth muscle antibody
Myosin-11 antibody
SMHC antibody
SMMHC antibody
smooth muscle isoform antibody
Smooth muscle myosin heavy chain 11 isoform SM2 antibody
Smooth muscle myosin heavy chain isoform SM2 antibody
计算分子量:227 kDa
配方:0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 5mg/ml Bovine Serum Albumin, 0.02% Sodium Azide
应用详情:WB: 1:50-1:200
IF:1:50-1:200
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文献和实验Isolation and Culture of Human Airway Smooth Muscle Cells
doublings and express smooth muscle cell-specific markers such as myosin heavy chain, myosin light chain kinase, α-actin, caldesmon, and SM22α . In addition, the synthetic function of these cells appears to be comparable to those studied in vivo
Myosin Light Chain Preparation
in the cold room and stir for 1 hour at 4℃. 11.Centrifuge at 8000 rpm for 20 minutes at 4℃ using the Beckman J21-C and 500 ml prechilled polycarb bottles with inner seals *usually 14 bottles- 3 spins are necessary;rotor JA10). 12.Filter the supernatant
Purification of Myosin Light Chain Kinase
7.0 4.Buffer C DE 52 Elution 10 mM MOPS- 5ml of 2M stock solution 1 liter 1 mM EDTA- 5ml of 0.2M stock solution 200 mM MaCl- 11.7g 1 mM DTT* - 1ml of 1M stock solution 0.1 mM PMSF* - 1.75ml of 10mg/ml stock solution pH 7.0 5.Buffer D Dialysis 10 mM MOPS
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