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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Alpha-1D Adrenergic Receptor.
- 形态:
liquid
- 保存条件:
Store at -20˚C
- 克隆性:
Polyclonal
- 适应物种:
Human
- 保质期:
12 months
- 抗原来源:
Rabbit
- 供应商:
南京赛戈巍生物科技有限公司
- 宿主:
Rabbit
- 应用范围:
WB, IHC, IF/ICC
- 靶点:
Uniprot:P25100
- 抗体英文名:
Alpha-1D Adrenergic Receptor Conjugated Antibody
- 规格:
50ul/100ul
计算分子量:60
配方:0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 5mg/ml Bovine Serum Albumin, 0.02% Sodium Azide
应用详情:WB: 1:50-1:200
IF:1:50-1:200
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文献和实验总蛋白(包括质膜和细胞器膜蛋白),但费时费力。目前,有一种最新型有效的膜蛋白提取工具,并具有高纯度、高特异性、高载量温和提取的优点。是基于1980年科学家在牛眼视网膜中发现的一种命名为Rho1D4的氨基酸序列以及其对应的抗体来作为亲和标签纯化膜蛋白取得了非常好的效果,将Rho1D4抗体链接在琼脂糖或磁珠上,用于亲和层析带有Rho1D4标签的膜蛋白,能近乎完美的解决这些研究难题。1) 什么是Rho1D4?Rho1D4是指在牛眼视网膜细胞内的牛视紫红质C端的最后九位氨基酸。Rho1D4得名于与该序列
In situ hybridisation to alpha satellite sequences (chromosome specific)
Alpha satellite sequences, whilst highly repetitive, are specific to each individual chromosome. These sequences flank the centromeres and can present a target measured in megabases. In this protocol a biotin or digoxigenin labelled DNA
Immunofluorescence: Rabbit Anti-Murine RELMα
for 25 minutes. 3. Incubate the tissue section with blocking buffer for 20 minutes. 4. Incubate the tissue section overnight at 4?C with Rabbit Anti-Murine RELMα at 4.0 ng/mL in 1X PBS with 0.01% Triton-X and 0.5% BSA. Wash the slide
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