- 询价
- Signalway Antibody
- 52691
- 2025年09月22日
- WB;ICC/IF;IHC
- Recombinant Rabbit
- Human;Mouse;Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
A synthetic phosphopeptide corresponding to residues surrounding Thr14 of human Cdk1/2
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Monoclonal antibody
- 适应物种：
Human;Mouse;Rat
- 保质期：
12 months
- 抗原来源：
Recombinant Rabbit
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
Recombinant Rabbit
- 应用范围：
WB;ICC/IF;IHC
- 靶点：
Uniprot：P06493 P24941
- 抗体英文名：
CDK1/2 (Phospho-Thr14) Rabbit mAb
- 规格：
50ul/100ul
计算分子量:34 kDa
配方:50nM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
应用详情:WB: 1:500-1:2000
ICC/IF: 1:50-1:200
IHC: 1:50-1:200
图片:
Western blot detection of Cdk1/2 (phospho-Thr14) in HepG2,U2OS,U251 using Cdk1/2 (phospho-Thr14) Rabbit mAb(1:1000 diluted)
,
Immunohistochemistry of Cdk1/2 (phospho-Thr14) in paraffin-embedded Human lung cancer tissue using Cdk1/2 (phospho-Thr14) Rabbit mAb at dilution 1/50
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文献和实验7.8 or Binding Buffer.(6) BD tubing 14-170-12F (Fisher).(7) Polyethylene tubing Clay Adanes.(8) 18 Gauge needles.(9) Binding Buffer (BioRad): make 1L. 314g/L ddH2O and filter through 0.22 um, filter. pH=9.0.(10) Elution buffer (BioRad): make 500 ml. 11g/500 ml
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
T-Cell Activation Using mAb to CD3
-0031 , or Purified, Cat. No. 14-0031 ) Complete RPMI-1640 Sterile single-cell suspension of mouse spleen or lymph nodes 96-well flat-bottom microtiter plates with lids (Costar Cat. No. 3596) MTT Buffer MTT Lysing Solution
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