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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
A synthetic phosphopeptide corresponding to residues surrounding Thr450 of human AKT1
- 形态:
liquid
- 保存条件:
Store at -20˚C
- 克隆性:
Monoclonal antibody
- 适应物种:
Human;Mouse;Rat
- 保质期:
12 months
- 抗原来源:
Recombinant Rabbit
- 供应商:
南京赛戈巍生物科技有限公司
- 宿主:
Recombinant Rabbit
- 应用范围:
WB,IHC
- 靶点:
Uniprot:P31749
- 抗体英文名:
AKT1 (Phospho-Thr450) Rabbit mAb
- 规格:
50ul/100ul
计算分子量:56 kDa
配方:50nM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
应用详情:WB: 1:500-1:2000
IHC: 1:50-1:200
图片:

Western blot detection of phospho-AKT1 (Thr450) in U2OS,C6 cell lysates using phospho-AKT1(Thr450) Rabbit mAb(1:1000 diluted).Predicted band size:56kDa.Observed band size:56kDa.
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文献和实验Monoclonal Antibodies to Cytochromes P450
specifically recognizes a single antigenic determinant or “epltope” on the protein antigen, which, in our studies, is a specific individual cytochrome P450 (P450). This specificity of the MAb continues for the lifetime of the hybridoma cell, which can be frozen
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
the phosphorylation site; therefore, substrate?directed, phosphorylation?state?sensitive, motif?specific (?phospho?motif?) antibodies represent powerful tools to identify novel kinase substrates and to investigate mechanisms of substrate phosphorylation
:使用 Anti-phospho-Akt (Ser473) Rabbit mAb 对石蜡包埋的人乳腺癌组织进行免疫组织化学分析。(图 A)使用免疫组化试剂盒M&R HRP/DAB Detection IHC Kit,抗体 1:100 稀释;(图 B) 采用普通免疫组化试剂盒,抗体 1:25 稀释。 图 6 免疫组化实验检测 Erk1/2 表达 注:使用 Anti-Erk1/2 Mouse mAb与p44/42 MAPK (Erk1/2)Rabbit mAb 对正常小鼠心脏组织进行免疫
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