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- 询价
- Signalway Antibody
- 13754
- 2025年09月22日
- WB,IHC-p,IF/ICC,ELISA
- Rabbit
- Human;Mouse;Rat
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- 文献和实验
- 技术资料
- 免疫原：
The antiserum was produced against synthesized peptide derived from human Lamin A/C around the phosphorylation site of Ser392. AA range:361-410
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Polyclonal
- 适应物种：
Human;Mouse;Rat
- 保质期：
12 months
- 抗原来源：
Rabbit
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
Rabbit
- 应用范围：
WB,IHC-p,IF/ICC,ELISA
- 靶点：
Uniprot：P02545
- 抗体英文名：
Lamin A/C (phospho-Ser392) Polyclonal Antibody
- 规格：
50ul/100ul
配方:Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
应用详情:Western Blot: 1/500 - 1/2000.
Immunohistochemistry: 1/100 - 1/300.
Immunofluorescence: 1/200 - 1/1000.
ELISA: 1/10000. Not yet tested in other applications.
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文献和实验Polyclonal and Monoclonal Antibodies
or O.N at 4¡C. 4) SECONDARY ANTIBODY a) Wash unbound antibody 4 times with PBS + 0.1% Tween 20. b) Add 100 ul of enzyme linked antibody to all wells. Do the appropriate dilutions in the Elisa buffer. (ex: HRP is 2000X). c
Polyclonal and Monoclonal Antibodies（克隆和克隆抗体）
at 4 C O.N. 3) Spin at 10,000 x g for 10 min. at 4 C to separate the serum. 4) Serum can be stored at -20 C after adding Glycerol to 50%. If monoclonal antibodies are desired, once you have a "good" polyclonal and a reliable screening method
Immunization Protocol for Monoclonal Antibody Production
Immunization Protocol for Monoclonal Antibody Production Antigens: Proteins: 10-100 µg Cells: 0.5 - 5.0 x107 Adjuvant: Complete Freunds Adjuvant
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