- 询价
- Signalway Antibody
- 55649
- 2025年09月22日
- WB,IHC,IF
- Rabbit
- Human;Mouse;Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein of human ERN1.
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Polyclonal
- 适应物种：
Human;Mouse;Rat
- 保质期：
12 months
- 抗原来源：
Rabbit
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
Rabbit
- 应用范围：
WB,IHC,IF
- 靶点：
Uniprot：O75460
- 抗体英文名：
IRE1 Rabbit pAb
- 规格：
50ul/100ul
计算分子量:6kDa/109kDa
配方:Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
应用详情:
WB1:500 - 1:2000
IHC1:50 - 1:200
IF/ICC1:50 - 1:200
图片:
Western blot analysis of extracts of various cell lines, using IRE1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
,
Immunohistochemistry of paraffin-embedded human breast cancer using IRE1 Rabbit pAbat dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
,
Immunofluorescence analysis of U2OS cells using IRE1 Rabbit pAb at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.
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文献和实验= NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific
GE Healthcare Benzamidine Sepharose™ 6B is p-aminobenzamidine covalently attached to Sepharose 6B by the epoxy coupling method. p-Aminobenzamidine (PAB), is a synthetic inhibitor of trypsin-like serine protease. Trypsin and trypsin
法所替代。利用微球菌核酸酶的切割特性（在染色质核小体之间的 DNA 更容易被切开），可以快速温和地将染色质 DNA 切断，且最大限度地保持了细胞原有染色质结构以及目的蛋白与 DNA 的结合状态，使 ChIP 的准确性和结果可信度大大提高。CST 推出的 SimpleChIP® 酶解法试剂盒，含有标准 ChIP 流程所需的整套试剂、ChIP 级蛋白质 G 微珠、阳性对照抗体（Histone H3(D2B12) XP® Rabbit mAb (ChIP Formulated) #4620）、阴性对照抗体、DNA
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