- 询价
- Signalway Antibody
- 54270
- 2025年09月22日
- WB,IHC,IF
- Rabbit
- Human;Mouse;Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein of human p27 KIP 1
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Polyclonal
- 适应物种：
Human;Mouse;Rat
- 保质期：
12 months
- 抗原来源：
Rabbit
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
Rabbit
- 应用范围：
WB,IHC,IF
- 靶点：
Uniprot：P46527
- 抗体英文名：
p27 KIP 1 Rabbit Polyclonal Antibody
- 规格：
50ul/100ul
计算分子量:22kDa
配方:Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
应用详情:WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
图片:
Immunofluorescence analysis of C6 cells using p27 KIP 1 Polyclonal at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
,
Immunofluorescence analysis of U-2 OS cells using p27 KIP 1 Polyclonal at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
,
Western blot analysis of extracts of various cell lines, using p27 KIP 1 at 1:1000 dilution.
,
Immunohistochemistry of paraffin-embedded human colon using p27 KIP 1 at dilution of 1:200 (40x lens).
,
Immunohistochemistry of paraffin-embedded rat liver using p27 KIP 1 at dilution of 1:200 (40x lens).
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文献和实验Polyclonal Antibody Production
Zealand White Rabbits and have them delivered to the ARC. Don’t put all your eggs in one basket only using one rabbit. Let them get accustomed to there home there for two weeks before the first pre-bleed. Pre-bleed
Polyclonal Antibody Production
Polyclonal Antibody Production Very useful for rapid and simple generation of antibodies for western blots, ELISA assays, and immunoprecipitation. Rabbit Immunization Initial Preparation Your antigen
then serially diluted in 100 µl assay buffer on the plate). Incubate @ RT for 1 hr while shaking. Meanwhile let TMB solution equilibrate to RT. Wash plate 3x and add 100 µl/well rabbit polyclonal anti-p27 antibody diluted 1:1000 in assay buffer
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