Actin,Smooth Muscle Antibody[P

平滑肌 smooth muscle，plain muscle

即无纹肌（ non-striated muscle）的通称。被视为较横纹肌原始的一种肌肉。平滑肌除作为无脊椎动物的躯体肌而有广泛分布外，在脊椎动物除心肌之外而大部分内脏肌也是由平滑肌组成的。虽有如斧足类的闭壳肌和足系牵引肌等是由平行走向长纤维状细胞（平滑肌纤维）所构成，但多数的平滑肌则是由长纺锤形（脊椎动物的内脏肌长不到 1毫米）的单核细胞构成。它不构成独立的器官，而只是成为构成体壁和内脏壁的因素（肌层）。其细胞实质仅由相当于横纹肌的向异性物质组成，整体表现同样的双折射。在有的平滑肌中可

LABELING MUSCLE ACTIN WITH IATR

,small vial. Procedure (perform under reduced light,4℃ unless otherwise noted) 1.Resuspend 10 mg lyophilized actin in 2 ml buffer 1.Be careful not to make bubbles. 2.Add DTT (100 mM stock)to 5 mM. 3.Dialyze against 250 ml buffer 1 overnight

Labeling Muscle Actin with 5-iodoacetamidofluorescein

with the dialysis buffer and read the OD at 495 nm. D/P = {OD495 x 41 / 60,000} / {(mg/ml)/ 43,000},should be 0.5-0.9. 3.Dilute to 3-5 mg/ml with the dialysis buffer.Calculate total mg of actin.Store as aliquots in liquid N2 after dissolving 2 mg sucrose per mg