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OGT Rabbit mAb[49799]

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  • 询价
  • Signalway Antibody已认证
  • 49799
  • 2025年09月23日
  • WB,ICC,IF,IHC,FC
  • Recombinant Rabbit
  • Human;Mouse;Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      Recombinant protein

    • 形态

      liquid

    • 保存条件

      Store at -20˚C

    • 克隆性

      Monoclonal antibody

    • 适应物种

      Human;Mouse;Rat

    • 保质期

      12 months

    • 抗原来源

      Recombinant Rabbit

    • 供应商

      南京赛戈巍生物科技有限公司

    • 宿主

      Recombinant Rabbit

    • 应用范围

      WB,ICC,IF,IHC,FC

    • 靶点

      Uniprot:O15294

    • 抗体英文名

      OGT Rabbit mAb

    • 规格

      50ul/100ul

    别名:FLJ23071 antibody
    GlcNAc transferase antibody
    HRNT1 antibody
    MGC22921 antibody
    O GlcNAc antibody
    O GlcNAc transferase p110 subunit antibody
    O GlcNAc transferase subunit p110 antibody
    O linked N acetylglucosamine (GlcNAc) transferase (UDP N acetylglucosamine:polypeptide N acetylglucosaminyl transferase) antibody
    O linked N acetylglucosamine (GlcNAc) transferase antibody
    O linked N acetylglucosamine transferase 110 kDa subunit antibody
    O-GlcNAc transferase subunit p110 antibody
    O-linked N-acetylglucosamine transferase 110 kDa subunit antibody
    ogt antibody
    OGT1_HUMAN antibody
    UDP N acetylglucosamine peptide N acetylglucosaminyltransferase 110 kDa subunit antibody
    UDP N acetylglucosamine peptide N acetylglucosaminyltransferase GlcNAc transferase antibody
    UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit antibody
    UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase antibody
    Uridinediphospho N acetylglucosamine:polypeptide beta N acetylglucosaminyl transferase antibody

    计算分子量:117 kDa
    配方:1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
    应用详情:WB: 1:500
    IHC: 1:50-1:200
    ICC: 1:500-1:1,000

    FC: 1:50-1:100
    图片:

    产品细节图片1

    Western blot analysis of OGT on Hela (1) and SiHa (2) cell lysate using anti-OGT antibody at 1/500 dilution.

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    产品细节图片2

    Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-OGT antibody. Counter stained with hematoxylin.

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    产品细节图片3

    Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-OGT antibody. Counter stained with hematoxylin.

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    产品细节图片4

    Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-OGT antibody. Counter stained with hematoxylin.

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    产品细节图片5

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-OGT antibody. Counter stained with hematoxylin.

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    产品细节图片6

    ICC staining OGT in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

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    产品细节图片7

    ICC staining OGT in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

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    产品细节图片8

    ICC staining OGT in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

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    产品细节图片9

    Flow cytometric analysis of Hela cells with OGT antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody


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    图标文献和实验
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    • Antibodies and Activity Measurements for the Detection of O-GlcNAc Transferase and Assay of its Substrate, UDP-GlcNAc

      focuses on protocols that are routinely used to analyze OGT expression and activity. First are detailed techniques using rabbit polyclonal anti-OGT antibodies, namely, Western blot, (co-)immunoprecipitation, and immunofluorescence

    • 三生三世,一场组化,一次豪赌

      h)即可。2. 修复大法——不仅仅是「煮一煮」微波炉修复:简单易行效果好,CST 推荐使用微波炉完成修复。合适的修复液:根据抗体说明书使用合适的修复液。用柠檬酸修复后,切片需浸泡在修复液中,自然冷却;而用 EDTA 修复后,切片可直接从修复缸中取出,直接进行下一步。注:使用不同的修复方式和不同生产商的抗体检测人肺癌组织中 EGFR 的表达。第一排为 CST 的 EGF Receptor (D38B1) XP® Rabbit mAb(#4267),EDTA 的修复方式明显优于柠檬酸盐及胃蛋白

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