- 询价
- Signalway Antibody
- 13356
- 2025年09月23日
- WB;ICC/IF;IHC
- Recombinant Rabbit
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Synthetic phospho-peptide corresponding to residues surrounding Ser9 of human GSK3 beta.
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Monoclonal
- 适应物种：
Human
- 保质期：
12 months
- 抗原来源：
Recombinant Rabbit
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
Recombinant Rabbit
- 应用范围：
WB;ICC/IF;IHC
- 靶点：
Uniprot：P49841
- 抗体英文名：
GSK3 beta(Phospho-Ser 9) Rabbit mAb
- 规格：
50ul/100ul
Glycogen synthase kinase-3 beta antibody
GSK 3 beta antibody
GSK-3 beta antibody
GSK3B antibody
GSK3B_HUMAN antibody
GSK3beta isoform antibody
Serine/threonine-protein kinase GSK3B antibody
计算分子量:47 kDa
配方:1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
应用详情:WB: 1:500-1:2000
ICC/IF: 1:50-1:200
IHC: 1:50-1:200
图片:
Western blot analysis of Phospho-GSK3 beta(Ser 9) on different lysates using anti-Phospho-GSK3 beta(Ser 9) antibody at 1/1,000 dilution. Positive control:
Lane 1: Hela
Lane 2: MCF-7
,
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Phospho-GSK3 beta(Ser 9) antibody. Counter stained with hematoxylin.
,
Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-Phospho-GSK3 beta(Ser 9) antibody. Counter stained with hematoxylin.
,
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-GSK3 beta(Ser 9) antibody. Counter stained with hematoxylin.
,
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Phospho-GSK3 beta(Ser 9) antibody. Counter stained with hematoxylin.
,
Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Phospho-GSK3 beta(Ser 9) antibody. Counter stained with hematoxylin.
,
ICC staining Phospho-GSK3 beta(Ser 9) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
,
ICC staining Phospho-GSK3 beta(Ser 9) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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文献和实验：使用 Anti-phospho-Akt (Ser473) Rabbit mAb 对石蜡包埋的人乳腺癌组织进行免疫组织化学分析。（图 A）使用免疫组化试剂盒M&R HRP/DAB Detection IHC Kit，抗体 1:100 稀释；(图 B) 采用普通免疫组化试剂盒，抗体 1:25 稀释。 图 6 免疫组化实验检测 Erk1/2 表达 注：使用 Anti-Erk1/2 Mouse mAb与p44/42 MAPK （Erk1/2）Rabbit mAb 对正常小鼠心脏组织进行免疫
at 10, 25, and 50 μM). At 24 h post-treatment, celllysates were obtained; SDS-PAGE and Western blotting were performed as described in Section 3, Methods. AR-A014418dose-dependent decreases of phospho-β-catenin (Ser33/37) and phospho-glycogen synthase
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
3.1 and 3.6 ). This will provide a “specific activity” measurement relative to total protein in the cell, and is themost accurate measure of total GSK3 activity, or2. Immunoblot using antibodies to phospho-Ser21 (GSK3a)or phospho-Ser9 (GSK3b) (see Sections 3.1 –
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