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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Synthesized Acetyl peptide derived from human E2F-1. at AA range: K117
- 形态:
liquid
- 保存条件:
Store at -20˚C
- 克隆性:
Polyclonal
- 适应物种:
Human;Mouse;Rat
- 保质期:
12 months
- 抗原来源:
Rabbit
- 供应商:
南京赛戈巍生物科技有限公司
- 宿主:
Rabbit
- 应用范围:
WB
- 靶点:
Uniprot:P09874
- 抗体英文名:
E2F-1 (Acetyl-K117) Polyclonal Antibody
- 规格:
50ul/100ul
计算分子量:70 kDa
配方:Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
应用详情:Western blotting: 1£º500-1£º2000
图片:

Western Blot analysis of 1,hela 2,mouse-brain cells using primary antibody diluted at 1:1000(˚C overnight). Secondary antibody£oGoat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25??C, 1 hour)
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文献和实验of the tryptic peptides derived from the purified enzyme revealed two amino acid sequences homologous to the 14-3-3 proteins. A polyclonal antibody was raised against purified ST-IV. A 33 kDa protein was co-immunoprecipitated from rat brain extracts with the anti
v468 Chapter 6 细胞内结肠腺瘤样息肉蛋白核定位以及细胞质检测
, and has characterized pathways for the trafficking of APC both into and out of the nucleus.Antibody specificity is an important factor in the determination of APC localization, and in this chapterwe outline a strategy for the unambiguous detection of APC using
) or MMS101R (BAbCo, Richmond, California). These antibody recognise cross-reacting yeast proteins of about 55kD or110kD, respectively, and can give a spotty background on immunofluorescence. Despite this drawback, the 3xHA tag has been used extensively
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