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- 2025年09月08日
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广州威佳科技有限公司
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文献和实验CSF Extract Prep for Spindle Assembly
back down. They should pack tightly after the jelly coat is removed. Wash 2-3 X in CSF-XB (150 ml total volume)- remove as much buffer as possible. Wash 2X in CSF-XB + 10 5g/ml PIs (100 ml total volume) Transfer into 1 ml of CSF-XB + PIs + 100 ug/ml
CSF Extract Prep for Spindle Assembly
. Wash 2-3 X in CSF-XB (150 ml total volume)- remove as much buffer as possible. Wash 2X in CSF-XB + 10 5g/ml PIs (100 ml total volume) Transfer into 1 ml of CSF-XB + PIs + 100 ug/ml cytochalasin D in 13 X 51 ultraclear tubes (let
back down. They should pack tightly after the jelly coat is removed. Wash 2-3 X in CSF-XB (150 ml total volume)- remove as much buffer as possible. Wash 2X in CSF-XB + 10 5g/ml PIs (100 ml total volume) Transfer into 1 ml of CSF-XB + PIs + 100 ug/ml
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