Mouse TNFa(Tumor Necrosis Factor Alpha) ELISA Kit
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Mouse TNFa(Tumor Necrosis Fact

or Alpha) ELISA Kit
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  • ¥1680 - 2400
  • ELK Biotechnology 科鹿生物已认证
  • 15.63-1000 pg/mL
  • 6.2 pg/mL
  • ELK1387
  • 2025年09月05日
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  • 企业认证

    • 详细信息
    • 文献和实验
    • 技术资料
    • 检测范围

      15.63-1000 pg/mL

    • 检测方法

      Sandwich

    • 灵敏度

      6.2 pg/mL

    • 规格

      48T/96T

    规格:48T产品价格:¥1680.0
    规格:96T产品价格:¥2400.0
    中文名称 小鼠肿瘤坏死因子α(TNFa)酶联免疫吸附检测试剂盒
    货号 ELK1387
    别称 TNF Alpha; DIF; TNF-A; TNFSF2; Cachectin; Tumor Necrosis Factor Ligand Superfamily Member 2
    试剂盒种类 常规法
    操作时间 3.5h
    样本体积 100μL
    可检测样本类型: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
    储存条件: 一、试剂盒拆封后可 4°C 保存 1 周,如果 1 周内未用完,请将剩余酶标板,冻干标准品,浓缩生物素化抗体,浓缩 HRP 酶结合物放置在-20°C 保存,其他试剂4°C 保存,并在6 个月内用完。
    二、若试剂盒未拆封:4°C(短时间存放,6 个月内有效);-20°C(长时间存放,1 年内有效);避免反复冻融,过期后请勿使用。
    三、 如果试剂盒整盒保存在-20°C,请在实验前一天晚上将试剂盒放置在4°C。
    试剂盒组成部分:
    中文名称 英文名称 规格 储存条件
    48T 96T
    酶标板(可拆) Pre-coated Microplate 6 条 x 8 孔 12 条 x 8 孔 4°C/-20°C
    冻干标准品 Standard (lyophilized) 1 2 4°C/-20°C
    标准品&样品稀释液 Standard/Sample Diluent Buffer 10 mL 20 mL 4°C/-20°C
    浓缩生物素化抗体(100×) Biotinylated Antibody (100×) 60 μL 120 μL 4°C/-20°C
    生物素化抗体稀释液 Biotinylated Antibody Diluent 6 mL 12 mL 4°C/-20°C
    浓缩HRP酶结合物(100×) Streptavidin-HRP (100×) 60 μL 120 μL 4°C/-20°C
    酶结合物稀释液 HRP Diluent 6 mL 12 mL 4°C/-20°C
    浓缩洗涤液(25×) Wash Buffer (25×) 10 mL 20 mL 4°C/-20°C
    显色底物溶液(TMB) TMB Substrate Solution 6 mL 10 mL 4°C/-20°C(避光)
    反应终止液 Stop Reagent 3 mL 6 mL 4°C/-20°C
    封板覆膜 Plate Covers 1 2 常温
    产品说明书 Instruction Manual 1份 1份 常温
    操作步骤:
    Mouse TNFa(Tumor Necrosis Fact 1. 试剂盒和样本室温平衡后,每孔加入标准工作缓冲液100 μL(按说明书梯度稀释)或样品100 μL, 37°C孵育80分钟。
    2. 弃去酶标板中液体,每孔加入200μL洗涤缓冲液,洗涤3次。 拍干后,每孔加入100μL 生物素化抗体工作液,37°C孵育50分钟。
    3. 弃去酶标板中液体,每孔加入200 μL洗涤缓冲液,洗涤3次。拍干后,每孔加入100 μL HRP酶工作液,37°C孵育50分钟。
    4. 弃去酶标板内液体,每孔加入200 μL洗涤缓冲液,洗涤5次。拍干后, 每孔加入90 μL TMB, 37℃孵育20 min 。
    5.每孔加入50 μL终止液,450 nm立即读数,计算结果。
    推荐标曲拟合信息: 见官网说明书详解
    微信扫码咨询客服:
    科鹿官方微信公众号:
    Mouse TNFa(Tumor Necrosis Fact       Mouse TNFa(Tumor Necrosis Fact

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    文献支持
    Mouse TNFa(Tumor Necrosis Factor Alpha) ELISA Kit
    ¥1680 - 2400