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见包装
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- 库存:
10
- 供应商:
广州市左克生物
- CAS号:
2020052-55-3
- 规格:
10mM*1mL/1mg/5mg/10mg
| 规格: | 10mM*1mL | 产品价格: | ¥3740.0 |
|---|---|---|---|
| 规格: | 1mg | 产品价格: | ¥935.0 |
| 规格: | 5mg | 产品价格: | ¥2329.0 |
| 规格: | 10mg | 产品价格: | ¥3731.0 |
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文献和实验2-Mercaptoethanol,5μM DM,10μM SB,10μM Y-27632); 神经分化培养基(主要成分:2% B27,1% GlutaMAX,20 ng/ml hEGF,20 ng/ml hFGF2,20 ng/ml hBDNF,20 ng/ml hNT3) 实验步骤 1、在涂有基质胶的平板上接种 hPSCs 或 iPSCs 细胞,并添加基础培养基进行培养,至出现明显细胞团; 2、将 30-50 个细胞团轻轻重悬于 12 ml 神经诱导培养基中,转移至 10cm 超低附着平板,将这一天记为第 0 天
Detection of Cyclins in Individual Cells by Flow and Laser Scanning Cytometry
Progression of cells through successive phases and checkpoints of the cell cycle is maintained by sequential phosphorylation of different sets of nuclear and cytoplasmic proteins by cyclin-dependent kinases (CDKs) (1 –12 ). By activating
spectrophotometer,SLM Instruments,Urbana,IL)at 530 nm.Monoformazan concentrations (and therefore 0,- concentrations)were calculated using an extinction coefficient of 12.8 mM-l cm-l.The NBT reduction rates are strictly linear with time up to 10 to 15 min
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