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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Peptide sequence around aa.1979~1983 (E-G-S-Q-S) derived from Human ATM.
- 形态:
liquid
- 保存条件:
Store at -20˚C
- 克隆性:
Polyclonal
- 适应物种:
Human
- 保质期:
12 months
- 抗原来源:
Rabbit
- 供应商:
南京赛戈巍生物科技有限公司
- 宿主:
Rabbit
- 应用范围:
WB IF
- 靶点:
Uniprot:Q13315
- 抗体英文名:
ATM Antibody
- 规格:
50ul/100ul
配方:Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
应用详情:Predicted MW: 350kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200
图片:

Western blot analysis of extracts from 293T cells using ATM(Ab-1981) Antibody #21147 and the same antibody preincubated with blocking peptide.
,

Immunofluorescence staining of methanol-fixed Hela cells using ATM(Ab-1981) Antibody #21147.
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文献和实验Analyzing the Regulation and Function of ATM
activity is measured using p531–44 -specific substrate or by immunoblotting extracts with an anti-phosphoserine 15 p53-specific antibody. Missense mutations affecting ATM kinase activity are detected using in vitro mutagenesis of ATM cDNA followed
hj841023 http://www.ncbi.nlm.nih.gov/nuccore/224514928?report=genbank&log$=seqview&from=11637691&to=11820525 刚开始着手实验前期工作,有很多不懂的,请教论坛里各位高手,如何看懂GenBank里的ATM基因DNA序列,以上是相关链接。谢谢各位指教了。:) freecell 请参考这个帖子: http
Expression and Assay of Recombinant ATM
A variety of genetic disorders involve genome instability and abnormal response to DNA damaging agents. Investigation of these disorders has revealed different metabolic pathways responsible for damage repair on one hand, and for signaling
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