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- 2025年11月27日
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文献和实验DNA Purification from Blood or Body Fluids
If the sample volume is less than 200 µl, add the appropriate volume of PBS. QIAamp Mini spin columns copurify RNA and DNA when both are present in the sample. RNA may inhibit some downstream enzymatic reactions, but not PCR. If RNA-free
E.Z.N.A.® Endo-free Plasmid Mini Kit I Spin Protocol
6-7 times. Incubate at room temperature for 1-2 minutes. 9. Transfer 700 u of the mixture(from Step 8) into a clean HiBindTM DNA Mini Column assembled in a 2 ml collection tube(provided). Centrifuge at 10,000 × g for 1 min at room temperature to pass
E.Z.N.A. Endo-Free Plasmid Mini Kit II Spin Protocol
with occasional mixing. Avoid vigorous mixing as this will shear chromosomal DNA and lower plasmid purity. (Store Solution II tightly capped when not in use.) 4. Add 250 ul ice-cold Buffer N3 and mix gently but throughly by inverting tube several times
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