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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG2a, kappa
- 保存条件:
-20℃
- 抗原来源:
Mouse
- 目录编号:
HA916013
- 供应商:
广州市左克生物
- 宿主:
Mouse
- 应用范围:
IHC, WB
- 靶点:
Bardet-Biedl syndrome 3 protein, BBS3, ADP-ribosylation factor-like protein 6, ARL6
- 抗体英文名:
Anti-BBS3/Arl6 Antibody (N302/10)
- 抗体名:
Anti-BBS3/Arl6 Antibody (N302/10)
- 规格:
50ug
产品简介:
品牌:abinscience
货号:HA916013
产品名称:Anti-BBS3/Arl6 Antibody (N302/10)
https://www.abinscience.com/product/34/17990.html
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文献和实验Monoclonal Antibody Production Protocol
the rinse twice. Add 100 ul of blocking solution to every well, leave 1 hr at room Temp or O.N at 4°C. PRIMARY ANTIBODY Add the antibody to be tested: Sup of cells = 25 ul, mix well by pipetting up and down (10 times).serum, ascites = 1:100 and a series
monoclonal antibody production fusion
, add ddH2 O up to 1 L Gluta x50, 1 L, pH 6.0 = glutamine, 10.8 g (1.48 mM) + HCl, 0.5N, 100 ml -> heat to 37ѓC, add ddH2 O up to 1 L aminopterine x100, 0.22-0.45 µm-filtered, store at -20ѓC = aminopterine, 4.4 mg (3.8µM)+ ddH2 O
Protocol for anti-HA antibody Western Blotting
of interest. 2) Transfer proteins in acrylamide gel to nylon membrane. 3) Block overnight @4℃ in PBS (or TBS, it doesn't matter) with 5% non-fat dry milk. I dilute 2.5 mg in 50 ml. 4) Wash off excess milk sol'n with PBS x3 .(important if you are re-using your antibody
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