- ￥1868
- abinScience
- CHINA
- MB674012
- 2025年09月04日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
- 保质期：
1 year
- 英文名：
Recombinant Mouse ADORA2A Protein, N-GST & C-His
- 库存：
999
- 供应商：
abinScience
- 规格：
100ug
| Product name | Recombinant Mouse ADORA2A Protein, N-GST & C-His |
|---|---|
| Catalog No. | MB674012 |
| Host species | \ |
| Species reactivity | \ |
| Immunogen | \ |
| Form | Lyophilized |
| Storage buffer | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Purity | >90% as determined by SDS-PAGE. |
| Clonality | \ |
| Isotype | \ |
| Applications | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Target | \ |
| Purification | \ |
| Endotoxin level | Please contact with the lab for this information. |
| Expression system | E. coli |
| Accession | Q60613 |
| Protein length | Ser64-Cys74&Asp98-Arg117&Asn141-Pro168&Arg194-Ser229 |
| Nature | Recombinant |
| Observed Molecular Weight | \ |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Alternative Names | Adenosine receptor A2a; Adora2a |
| Clone ID | \ |
| Species | Mus musculus (Mouse) |
| Product Usage Information | \ |
| Note | For research use only |
abinScience, founded in 2023 in Strasbourg, France, is committed to developing and producing high-quality life science reagents. Rooted in one of Europe’s leading hubs for scientific innovation, abinScience empowers global research through reliable, efficient experimental solutions. Guided by the vision of “Empowering Bioscience Discovery,” we support scientists worldwide in advancing the frontiers of bioscience.
Technology and Innovation
Relying on the global leading position in antibody and protein research and more than 20 years of industry experience of its parent company ProteoGenix since 2003, abinScience has inherited advanced technologies and high-quality standards. In particular, the XtenCHO™ system developed by ProteoGenix, which has two modes of transient expression and stable expression, has been widely recognized as a core technology to improve the efficiency of protein production, providing a solid foundation for ab...
Product categories and application fields.
Main products.
10000+ high-activity proteins.
15000+ antibody series products:
Classic monoclonal antibody.
Monoclonal antibody
Internal control antibody, labeled antibody.
High-quality phosphorylation antibodies.
Highly specific nanobodies and small molecule antibodies.
Functional antibodies in vivo.
Low-background flow cytometry antibodies.
DNA/RNA antibodies and PEG antibodies.
1000+ reagent kit series products.
Large-scale packaging customization services: We offer personalized antibody design, protein production, molecular optimization, and experimental development services.
Application field
Infectious disease research: including viruses, superbugs, parasites, etc.
Neuroscience research: Supporting research on neurodegenerative diseases such as Alzheimer's disease and Parkinson's disease.
Immunology research: Covers the research needs of immune activation, inhibition, and allergies and abnormal reactions.
Oncology research: Supporting the development of tumor biology and anti-tumor drugs.
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文献和实验• International society of sports nutrition position stand: caffeine and exercise performance., PMID:33388079
• Endothelial adenosine A2a receptor-mediated glycolysis is essential for pathological retinal angiogenesis., PMID:28928465
• Inhibition of adenosine/A2A receptor signaling suppresses dermal fibrosis by enhancing fatty acid oxidation., PMID:40301970
• Adenosine receptor A2a blockade by caffeine increases IFN-gamma production in Th1 cells from patients with rheumatoid arthritis., PMID:35023427
• Regulation of sleep homeostasis mediator adenosine by basal forebrain glutamatergic neurons., PMID:32883833
• The adenosine-A2a receptor regulates the radioresistance of gastric cancer via PI3K-AKT-mTOR pathway., PMID:35122587
• Critical role for adenosine receptor A2a in β-cell proliferation., PMID:27818940
• Adenosine Plasma Level and A2A Receptor Expression in Patients With Cardiogenic Shock., PMID:29923934
• Evaluation of Adenosine A2A receptor gene polymorphisms as risk factors of methamphetamine use disorder susceptibility and predictors of craving degree., PMID:35987070
depends on product and its function. N-terminal sequencing revealed that the purified rBoNTC(Hc )-N is missing the first eight amino acids of the N-terminus of the protein, whereas the purified rBoNTC(Hc )-C protein is intact. After a mouse bioassay test
Thrombin Cleavage of GST-Fusion protein
activity keeps the enzyme in aliquots at -80°C. Thrombin (MW 37kDa) ~4500units/mg. One unit cleaves theoretical 100ug protein in 16hr at 22°C in buffer. Another possibility is to use high purity enzyme from Sigma # T-6884: 3-10 U of thrombin can be used
5- If required, bring to 1pM RARE or any HRE you need 6- Incubate 30min at 4 _ C 7- Add 50_ L of the GST affinity matrix 8- Incubate for 2 hours at 4 _ C 9- Wash twice with PBS-DTT buffer (quick pulse to 500 x g) 10- Add 25_ L of SDS loading buffer directly
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