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- 禾午生物
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- 2025年08月31日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
99
- 英文名：
pLV3-CMV-F2(mouse)-6×His-Puro
- 保质期：
3个月~12个月
- 供应商：
上海禾午生物有限公司
- 保存条件：
负20℃＆负80℃
- 规格：
干粉＆甘油菌
| 名称 | pLV3-CMV-F2(mouse)-6×His-Puro |
| 别称 | NM_010168.4;Cf-2; Cf2; FII |
| 基因长 | 1854bp |
| 质粒宿主 | 哺乳细胞,慢病毒 |
| 质粒用途 | 蛋白表达 |
| 片段类型 | ORF |
| 片段物种 | 小鼠 |
| 原核抗性 | Amp |
| 筛选标记 | Puro |
| 启动子 | CMV |
| 复制子 | pUC |
| 感受态 | Stbl3 |
| 温度 | 37度 |
| 背景载体 | pLV3-CMV-MCS-6×His-Puro |
| 正向引物 | CMV-F |
| 拷贝数 | 高 |
| pLV3-CMV-CD3D(human)-3×FLAG-Puro |
| pLV3-CMV-CD6(human)-3×FLAG-Puro |
| pLV3-CMV-SEC14L5(human)-3×FLAG-mCherry-Puro |
| pLV3-CMV-CD247(human)-3×FLAG-Puro |
| pLV3-CMV-F2(mouse)-6×His-Puro |
| pLV3-CMV-PLG(human)-6×His-Puro |
| pLV3-CMV-EIF4A3(human)-3×HA-CopGFP-Puro |
| pLV3-CMV-SLC31A1(human)-CopGFP-Puro |
| pLV3-CMV-Fth1(mouse)-CopGFP-Puro |
| pLV3-CMV-EGFP-PON1(human)-Puro |
| pLV3-CMV-GFPT2(human)-EF1a-mCherry-Puro |
| pHAGE-EF1a-COL8A1(human)-IRES-ZsGreen1 |
| pLV2-CMV-Sox10(mouse)-AcGFP1-Puro |
| pLV3-CMV-Vhl(mouse)-3×HA-EF1a-mCherry-Puro |
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文献和实验Transgenic Mouse and Transgenic Rat Outline
sequences in not required for BAC transgenes. Transgene constructs are then quantitated and microinjected into (C57BL/6 X SJL)F2 mouse eggs and surgically transferred to recipients. This is provided on a fee for service basis by the Transgenic Facility
Blastocyst embryo transfer into pseudopregnant recipient female mouse（图）
and exhibit bad planes of anesthesia when heavy. This is also a very inexpensive mouse to use. As an alternate, another strain I have used with considerable success is B6D2F1. These mice are hardy and display hybrid vigor. The Equipment For surgery
PROTOCOL General anesthesia inject i.p. 250µl/mouse of the ANESTHETIC mixture for verify the anesthetic effect check plantar reflexes (animal must be unresponsive to squeezing hind feet) Preparation
技术资料