Ms Foxp3 PE-CF594 3G3 100ug

产品概述

Ms Foxp3 PE-CF594 3G3 100ug 为 BD Pharmingen 品牌流式抗体，货号 567456，靶向 Foxp3，荧光标记 PE-CF594，克隆 3G3 (RUO)，规格 100 ug。适用于 Intracellular staining (flow cytometry) (Routinely Tested) 检测，仅供科研使用（RUO）。

靶点与抗体说明

The 3G3 monoclonal antibody specifically binds to Forkhead box protein P3 (Foxp3) which is also known as Scurfin and JM2. Foxp3 is a 50-55 kDa protein that is encoded by Foxp3 (forkhead box P3) which belongs to the forkhead/winged helix family of transcriptional regulators. FoxP3 contains a single C2H2-type zinc-finger motif, a leucine-zipper region, and a C-terminal forkhead DNA-binding domain. Foxp3 is expressed by natural (nTreg) and induced/adaptive (iTreg) T regulatory cells. Foxp3 is a key transcription factor for Treg cell development and regulatory function. Treg cells play crucial roles in maintaining immune homeostasis by enforcing immunological tolerance to self-antigens and by suppressing excessive responses made by other immune cells to foreign antigens. Ectopic expression of Foxp3 in conventional T cells is sufficient to induce suppressive activity, repress the production of cytokines such as IL2 and IFN-γ, and upregulate Treg cell-associated molecules such as CTLA4/CD152 and GITR/TNFRSF18. A mutation in Foxp3 causes a lack of functional Tregs in Scurfy (sf) mice which develop a systemic disease state with autoimmune manifestations. The 3G3 antibody reportedly binds to the N-terminus of Foxp3.

核心参数

荧光染料信息

PE-CF594 BD Horizon™ PE-CF594 dye is a part of the BD PE family of dyes. This tandem fluorochrome is comprised of a R-Phycoerythrin (PE) donor that has excitation maxima (Ex Max) of 496-nm and 566-nm and an acceptor dye with an emission maximum (Em Max) at 615-nm. PE-CF594, driven by BD innovation, is designed to be excited by the blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and detected using an optical filter centered near 615 nm (e.g., a 610/20-nm bandpass filter). The donor dye can be excited by the Blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and the acceptor dye can be excited by the green (532-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye. 格式:PE-CF594最多激发源:496 nm, 566 nm最多发射源:615 nm

保存与运输

建议 2-8℃ 避光保存，请勿冷冻，避免反复冻融。 存储缓冲液：Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

参考文献

Brunkow ME, Jeffery EW, Hjerrild KA, et al. Disruption of a new forkhead/winged-helix protein, scurfin, results in the fatal lymphoproliferative disorder of the scurfy mouse. Nat Genet. 2001; 27(1):68-73. (Biology). Gavin MA, Torgerson TR, Houston E, et al. Single-cell analysis of normal and FOXP3-mutant human T cells: FOXP3 expression without regulatory T cell development. Proc Natl Acad Sci U S A. 2006; 103(17):6659-6664. (Immunogen: Flow cytometry). Hadaschik EN, Wei X, Leiss H, et al. Regulatory T cell-deficient scurfy mice develop systemic autoimmune features resembling lupus-like disease. Arthritis Res Ther. 2015; 17(35):1-12. (Biology). Law JP, Hirschkorn DF, Owen RE, Biswas HH, Norris PJ, Lanteri MC. The importance of Foxp3 antibody and fixation/permeabilization buffer combinations in identifying CD4+CD25+Foxp3+ regulatory T cells. Cytometry A. 2009; 75(12):1040-1050. (Clone-specific: Flow cytometry). Mailer RKW. Alternative Splicing of FOXP3-Virtue and Vice. Front Immunol. 2018; 9(530):1-11. (Clone-specific).