Hu CD86 BV711 IT2.2 100Tst

产品概述

Hu CD86 BV711 IT2.2 100Tst 为 BD Pharmingen 品牌流式抗体，货号 569383，靶向 CD86，荧光标记 BV711，克隆 IT2.2 (RUO)，规格 100 Tests。适用于 Flow cytometry (Routinely Tested) 检测，仅供科研使用（RUO）。

靶点与抗体说明

The IT2.2 monoclonal antibody specifically binds to CD86. CD86 is a ~ 75 kDa type I transmembrane glycoprotein which is also known as B7-2 or B70. CD86 is primarily expressed on monocytes, dendritic cells, Langerhans cells, and activated B cells including B lymphoid cells in germinal centers and Epstein-Barr virus transformed B-cell lines. CD86 is a ligand for CD28 and CD152 (CTLA-4) and may play an important role in costimulation of T cells in primary immune responses. Competitive binding assays demonstrate that, while both the IT2.2 and FUN-1 monoclonal antibodies recognize the same CD86 molecule, they react with different epitopes. IT2.2 blocks the costimulation activity of CD86 in functional studies and blocks binding of human CTLA-4-Ig fusion protein to CD86 gene-transfected cells.

核心参数

荧光染料信息

BV711 The BD Horizon Brilliant Violet™ 711 (BV711) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This tandem fluorochrome is comprised of a BV421 donor with an excitation maximum (Ex Max) of 407-nm and an acceptor dye with an emission maximum (Em Max) at 713-nm. BV711, driven by BD innovation, is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 710-nm (e.g., a 712/20-nm bandpass filter). The acceptor dye can be excited by the Red (628–640-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye. 格式:BV711最多激发源:407 nm最多发射源:713 nm

保存与运输

建议 2-8℃ 避光保存，请勿冷冻，避免反复冻融。 存储缓冲液：Aqueous buffered solution containing ≤0.09% sodium azide.

参考文献

Azuma M, Ito D, Yagita H, et al. B70 antigen is a second ligand for CTLA-4 and CD28.. Nature. 1993; 366(6450):76-9. (Immunogen: Blocking, Flow cytometry). Engel P, Gribben JG, Freeman GJ, et al. The B7-2 (B70) costimulatory molecule expressed by monocytes and activated B lymphocytes is the CD86 differentiation antigen. Blood. 1994; 84(5):1402-1407. (Biology). Engel P, Wagner N, Tedder TF. CD86 Workshop Report. In: Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995:703-705. Hardie DL, Casamayor M, Johnson GD, et al. CD86 Workshop Panel report. In: Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997:201-204. Nozawa Y, Wachi E, Tominaga K, Abe M, Wakasa H. A novel monoclonal antibody (FUN-1) identifies an activation antigen in cells of the B-cell lineage and Reed-Sternberg cells. J Pathol. 1993; 169(3):309-315. (Biology).