Hu Neuropilin-1 BB515 U21-1283

产品概述

Hu Neuropilin-1 BB515 U21-1283 100Tst 为 BD Pharmingen 品牌流式抗体，货号 566036，靶向 CD304，荧光标记 BB515，克隆 U21-1283 (RUO)，规格 100 Tests。适用于 Flow cytometry (Routinely Tested) 检测，仅供科研使用（RUO）。

靶点与抗体说明

The U21-1283 monoclonal antibody specifically recognizes Neuropilin-1 (NRP1), also known as CD304, Blood dendritic cell antigen 4 (BDCA4), and Vascular endothelial cell growth factor 165 receptor (VEGF165R). CD304 is a type I transmembrane glycoprotein involved in the development of the nervous and cardiovascular systems. It mediates the interaction, growth, survival, and migration of a variety of normal and tumor cells. CD304 is expressed on neurons, thymocytes, regulatory T cells, a subset of T follicular helper cells, dendritic cells, endothelial cells, and certain tumor cells. Neuropilin-1 has a very short cytoplasmic domain and interacts with various coreceptors to form ligand-binding, signal-transducing receptor complexes. CD304 complexes with Plexin-A family members to bind chemorepellent Class 3 Semaphorins and guide neuronal axon growth. It also functions as a coreceptor with VEGFR2/CD309 to stimulate angiogenesis in response to VEGF165. CD304 mediates the interactions between some T cells and dendritic cells. The antibody was conjugated to BD Horizon BB515 which is part of the BD Horizon Brilliant™ Blue family of dyes. With an Ex Max near 490 nm and an Em Max near 515 nm, BD Horizon BB515 can be excited by the blue laser (488 nm) laser and detected with a 530/30 nm filt

核心参数

荧光染料信息

BB515 The BD Horizon Brilliant™ Blue 515 (BB515) dye is part of the BD Horizon Brilliant™ Blue family of dyes. This dye is a polymer fluorochrome with an excitation maximum (Ex Max) at 490-nm and an emission maximum (Em Max) of 515-nm. Driven by BD innovation, BB515 is designed to be excited by the blue laser (488-nm) and detected using an optical filter centered near 520-nm (e.g., 530/30-nm). BB515 reagents are significantly brighter than equivalent FITC or Alexa Fluor™ 488 reagents with less spillover into the PE detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye. 格式:BB515最多激发源:490 nm最多发射源:515 nm

保存与运输

建议 2-8℃ 避光保存，请勿冷冻，避免反复冻融。 存储缓冲液：Aqueous buffered solution containing ≤0.09% sodium azide.

参考文献

Bruder D et al. Neuropilin-1: a surface marker of regulatory T cells.. Eur J Immunol. 2004; 34(3):623-630. (Biology). Dzionek A et al. BDCA-2, BDCA-3, and BDCA-4: three markers for distinct subsets of dendritic cells in human peripheral blood.. J Immunol. 2000; 165(11):6037-6046. (Biology). Mizui M, Kumanogoh A, Kikutani H. Immune semaphorins: novel features of neural guidance molecules.. J Clin Immunol. 2009; 29(1):1-11. (Biology). Pan Q et al. Neuropilin-1 binds to VEGF121 and regulates endothelial cell migration and sprouting.. J Biol Chem. 2007; 282(33):24049-24056. (Biology). Renand A, Milpied P, Rossignol J, et al. Neuropilin-1 expression characterizes T follicular helper (Tfh) cells activated during B cell differentiation in human secondary lymphoid organs.. PLoS ONE. 2013; 8(12):e85589. (Biology). Soker S, Takashima S, Miao HQ, Neufeld G, Klagsbrun M. Neuropilin-1 is expressed by endothelial and tumor cells as an isoform-specific receptor for vascular endothelial growth factor.. Cell. 1998; 92(6):735-45. (Biology).