Hu Siglec-9 (CD329) R718 K8 25

产品概述

Hu Siglec-9 (CD329) R718 K8 25Tst 为 BD Pharmingen 品牌流式抗体，货号 569485，靶向 CD329 (Siglec-9)，荧光标记 R718，克隆 K8 (RUO)，规格 25 Tests。适用于 Flow cytometry (Routinely Tested) 检测，仅供科研使用（RUO）。

靶点与抗体说明

The K8 monoclonal antibody specifically recognizes Sialic acid-binding Ig-like lectin 9 (Siglec-9) which is also known as CD329. Siglec-9 (CD329) is a single-pass type I transmembrane glycoprotein that is encoded by SIGLEC9 which belongs to the I-type lectins within the Ig superfamily. This lectin is comprised of an N-terminal extracellular region that contains an IgV domain, which binds sialic acid, followed by two Ig-like C2-type domains, a transmembrane region, and an intracellular domain with an immunoreceptor tyrosine-based inhibitory motif (ITIM) and an ITIM-like motif . It is highly expressed on monocytes, neutrophils, and expressed at lower levels on a proportion of NK cells, B cells and T cells. Siglec-9 (CD329) may play roles in the regulation of T cell and NK cell responses and neutrophil apoptosis.

核心参数

荧光染料信息

R718 The BD Horizon™ Red 718 (R718) Dye is part of the BD red family of dyes. It is a small organic fluorochrome with an excitation maximum (Ex Max) at 695-nm and an emission maximum (Em Max) at 718-nm. Driven by BD innovation, R718 is designed to be excited by the red laser (627–640-nm) and detected using an optical filter centered near 720-nm (e.g., a 720/40-nm bandpass filter). R718 is a brighter alternative to Alexa Fluor™ 700. R718 is also a bright small molecule alternative to APC-R700 with lower spread into the APC detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye. 格式:R718最多激发源:695 nm最多发射源:718 nm

保存与运输

建议 2-8℃ 避光保存，请勿冷冻，避免反复冻融。 存储缓冲液：Aqueous buffered solution containing ≤0.09% sodium azide.

参考文献

Avril T, Floyd H, Lopez F, Vivier E, Crocker PR. The membrane-proximal immunoreceptor tyrosine-based inhibitory motif is critical for the inhibitory signaling mediated by Siglecs-7 and -9, CD33-related Siglecs expressed on human monocytes and NK cells.. J Immunol. 2004; 173(11):6841-9. (Clone-specific: Flow cytometry, Functional assay). Carlin AF, Uchiyama S, Chang YC, Lewis AL, Nizet V, Varki A. Molecular mimicry of host sialylated glycans allows a bacterial pathogen to engage neutrophil Siglec-9 and dampen the innate immune response. Blood. 2009; 113(14):3333-3336. (Biology). Fraschilla I, Pillai S. Viewing Siglecs through the lens of tumor immunology.. Immunol Rev. 2017; 276(1):178-191. (Biology). Nguyen DH, Hurtado-Ziola N, Gagneux P, Varki A. Loss of Siglec expression on T lymphocytes during human evolution.. Proc Natl Acad Sci U S A. 2006; 103(20):7765-70. (Biology). Zhang JQ, Nicoll G, Jones C, Crocker PR. Siglec-9, a novel sialic acid binding member of the immunoglobulin superfamily expressed broadly on human blood leukocytes.. J Biol Chem. 2000; 275(29):22121-6. (Immunogen: Flow cytometry).