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- 文献和实验
- 技术资料
- 供应商:
BD Pharmingen
- 级别:
RUO
- 克隆性:
单克隆
- 抗原来源:
Mouse
- 标记物:
PE-Cy7
- 宿主:
Mouse
- 适应物种:
Mouse
- 亚型:
Mouse IgG1, κ
- 形态:
液体
- 应用范围:
Flow Cytometry (FCM)
- 保存条件:
2-8℃避光保存
- 规格:
100 Tests
产品概述
Ms IgG1 Kpa ItCl PE-Cy7 MOPC-21 100Tst 为 BD Pharmingen 品牌流式抗体,货号 557872,荧光标记 PE-Cy7,克隆 MOPC-21 (also known as MOPC21; MOPC 21) (RUO),规格 100 Tests。适用于 Flow cytometry, Isotype control (Routinely Tested), Intracellular staining (flow cytometry) (Tested During Development) 检测,仅供科研使用(RUO)。
靶点与抗体说明
The MOPC-21 immunoglobulin is a mouse myeloma protein. The MOPC-21 immunoglobulin was selected as an isotype control following screening for low background on a variety of mouse and human tissues.
核心参数
| 货号 | 557872 |
| 品牌 | BD Pharmingen |
| 克隆号 | 克隆 MOPC-21 (also known as MOPC21; MOPC 21) (RUO) |
| 宿主 | Mouse |
| 同种型 | Mouse IgG1, κ |
| 荧光标记 | PE-Cy7 |
| 反应种属 | Mouse |
| 验证应用 | Flow cytometry, Isotype control (Routinely Tested), Intracellular staining (flow cytometry) (Tested During Development) |
| 推荐用量 | 5 µl |
| 规格 | 100 Tests |
| 存储缓冲液 | Aqueous buffered solution containing BSA and ≤0.09% sodium azide. |
荧光染料信息
PE-Cy7 PE-Cy7 dye is a part of the BD PE family of dyes. This tandem fluorochrome is comprised of a R-Phycoerythrin (PE) donor that has excitation maxima (Ex Max) of 496-nm and 566-nm and an acceptor dye, Cy™7, with an emission maximum (Em Max) at 781-nm. PE can be excited by the Blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and detected using an optical filter centered near 781 nm (e.g., a 760/60-nm bandpass filter). The donor dye can be excited by the Blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and the acceptor dye can be excited by the Red (627–640-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye. 格式:PE-Cy7最多激发源:496 nm, 566 nm最多发射源:781 nm
保存与运输
建议 2-8℃ 避光保存,请勿冷冻,避免反复冻融。 存储缓冲液:Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
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文献和实验Elabscience|肺泡巨噬细胞研究必看:LPS诱导急性肺炎模型制备与检测全攻略
抗体[X54-5/7.1] E-AB-F09792C FITC 标记小鼠IgG1,κ同型对照抗体[MOPC-21] E-AB-F1120D PE 标记抗小鼠Ly-6G/Ly-6C(Gr-1)抗体[RB6-8C5] E-AB-F0991H PE/Cyanine7 标记抗小鼠CD11c抗体[N418] AN00629J PerCP/Cyanine5.5 标记抗小鼠Siglec-F/CD170抗体[S17007L] E-AB-F0997A 纯化抗小鼠CD16/32抗体[2.4G
水分(%)≤5.0,蛋白质(%) 14――16,碳水化合物(%)55――65 ,脂肪(%)18――21,灵芝多糖(mg/l00g)100――120,有机锗(mg/100g)5――8,维生素A(1U/100g)2000――2200,维生素E(1U/100g)10――12,维生素C(mg/100g)40――60,钙(mg/100g)600――700,铁(mg/100g)8――10,锌(mg/100g)6――8。 3.微生物指标 细菌总数(个/g)≤30000,大肠菌群(个/100
,那么它的isotype 是mouse IgG2a, 所以可以用purified(纯化的) mouse IgG2a来做OX-42的同型对照(Isotype Control)。一般的的生物技术公司和国内的代理都有出售。 同型对照:是指与MoAb相同的、未免疫小鼠的免疫球蛋白亚类,若使用直接免疫荧光染色法,同型对照也应标记荧光色素,如IgG1 FITC、IgG2a、PE等。主要考虑了细胞的自发荧光、FC受体介导的抗体结合和非特异性抗体结合等影响因素。此外,同型对照与MoAb所标记的荧光色素、浓度、F:P比值(标记的荧
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