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Recombinant Human Ubiquitin Gl

y76 to Ala76 mutation
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  • ¥987 - 2457
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  • abs44074626
  • 2025年08月28日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件

      Store vial at -20°C to -80°C. When stored at the recommended temperature, this protein is stable for 12 months. Please prevent freeze-thaw cycles.

    • 保质期

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    • 英文名

      Recombinant Human Ubiquitin Gly76 to Ala76 mutation

    • 库存

      999

    • 供应商

      Absin

    • 规格

      5ug/20ug

    规格:5ug产品价格:¥987.0
    规格:20ug产品价格:¥2457.0

    产品名:

    Recombinant Human Ubiquitin Gly76 to Ala76 mutation

    品牌:

    Absin

    货号:

    abs44074626

    保持条件:

    Store vial at -20°C to -80°C. When stored at the recommended temperature, this protein is stable for 12 months. Please prevent freeze-thaw cycles.

    浓度:

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    免疫原:

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    抗原名称:

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    使用方法:

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    产品描述:

        The conserved 76 amino acid protein ubiquitin (Ub) regulates a host of intracellular processes through its enzymatic conjugation to other cellular proteins.
    Ubiquitination occurs through sequential steps catalyzed by activating (E1), conjugating (E2), and ligase (E3) enzymes. The final step results in the formation of an isopeptide bond between Ub’s C-terminal glycine residue (G76) and a lysine residue of the target protein, although N-terminal ubiquitination is also known.
    Outcomes of this modification include destabilization of the conjugated protein, altered protein trafficking and functional modulation.
    After targeting the protein for specific localizations, ubiquitin is released from the substrate by deubiquitinating enzymes.
    A mutant ubiquitin, having a Gly to Ala substitution at the C-terminus (G76A ubiquitin) supported several downstream reactions of the proteolytic pathway but inhibits the deubiquitination process.
    As consequence, the Ub derivative becomes irreversibly conjugated to protein, shifting the equilibrium between the bound and unbound form in the direction of conjugation, at the expense of the free form.
     
        Recombinant human ubiquitin featuring a Gly76 to Ala76 mutation that, by inhibiting the ubiquitin hydrolases, prevents the removal of ubiquitin from protein ubiquitin conjugates. 
        Ubiquitin G76A is expressed in E.coliand purified by ion-exchange chromatography.

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    图标文献和实验
    相关实验
    • Human Mutation Databases

        Figure 1.10.3 Page from the Human Gene Mutation Database (HGMD) showing entries for the phenylalanine hydroxylase ( PAH ) gene by mutation type and phenotype.

    • Selection of a Platform for Mutation Detection

      here is on applications involving human disease, many of these platforms can be easily adapted to the study of other organisms. Curr. Protoc. Hum. Genet. 56:7.15.1?7.15.30. © 2008 by John Wiley & Sons, Inc. Keywords: mutation detection; mutation

    • Discovery Genetics: The History and Future of Spontaneous Mutation Research

      , D. 2001. The mouse Hoxd13(spdh) mutation, a polyalanine expansion similar to human type II synpolydactyly (SPD), disrupts the function but not the expression of other Hoxd genes. Dev. Biol. 237:345‐353.

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