重组人4-1BB/CD137蛋白（PE定点偶联，His标签）

重组人4-1BB/CD137蛋白（PE定点偶联，His标签）（产品说明）
蛋白名称：Human 4-1BB/CD137 Protein (Site-Specific PE Conjugation, His Tag)
蛋白构建：A DNA sequence encoding the human 4-1BB/CD137 (NP_001552.2) (Met 1-Gln 186) was expressed with a C-terminal polyhistidine tag. The protein is site-specifically conjugated with PE (Excitation= 495 - 566 nm, Emission Max.= 575 nm).
表达宿主：HEK293 Cells
蛋白活性：1.Flow cytometric analysis of anti-CD137 CAR expression. 5e5 of anti-CD137 CAR-293 cells were stained with 100 μL of 1:100 (1 μL stock solution in 100 μL FACS buffer) of PE-conjugated human CD137 Protein (Site-Specific PE Conjugation, His Tag, Cat. No. 10041-H86H-SP) and negative control protein. Non-transduced 293 cells were used as a control (left). PE signal was used to evaluate the binding activity (QC tested). 2.Binding activity of PE-conjugated CD137 protein to PBMC cells. PBMC cells were stained with FITC conjugated anti-CD3 antibody and PE-conjugated human CD137 Protein (Site-Specific PE Conjugation, His Tag, Cat. No. 10041-H86H-SP) and detected by flow cytometry. FITC signal was used to evaluate the content of CD3+ T cells in PBMCs. PE signal was used to evaluate the non-specific binding activity to PBMCs (QC tested).
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Leu 24
蛋白分子量：The protein has a predicted molecular mass of 20.8 kDa.
蛋白NP号：NP_001552.2
蛋白氨基酸序列：Met1-Gln186