- ￥1500 - 2300
- Absin
- abs136414
- 上海
- 2025年08月21日
- WB: 1:500-1:2000, IHC: 1:50-1:200, IF: 1:100-1:500, ELISA(peptide): 1:20000-1:40000
- Rabbit
- Human;Mouse;Rat
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
Rabbit anti-IL10 Polyclonal mAb
- 抗体英文名：
Rabbit anti-IL10 Polyclonal Antibody
- 靶点：
见优宁维官网
- 浓度：
1mg/ml
- 应用范围：
WB: 1:500-1:2000, IHC: 1:50-1:200, IF: 1:100-1:500, ELISA(peptide): 1:20000-1:40000
- 宿主：
Rabbit
- 适应物种：
Human;Mouse;Rat
- 保质期：
见优宁维官网
- 抗原来源：
见优宁维官网
- 级别：
优级
- 库存：
999
- 供应商：
Absin
- 标记物：
Unlabeled
- 克隆性：
见优宁维官网
- 保存条件：
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
- 免疫原：
A synthesized peptide derived from Human IL10(Accession P22301), corresponding to amino acid residues G113-V142.
- 规格：
50ug/100ug
| 规格： | 50ug | 产品价格： | ￥1500.0 |
|---|---|---|---|
| 规格： | 100ug | 产品价格： | ￥2300.0 |
别名：兔抗IL10多克隆抗体
免疫原：A synthesized peptide derived from Human IL10(Accession P22301), corresponding to amino acid residues G113-V142.
应用：WB: 1:500-1:2000, IHC: 1:50-1:200, IF: 1:100-1:500, ELISA(peptide): 1:20000-1:40000
保持条件：Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
使用方法：WB,IHC,IF,ELISA
标记：Unlabeled
宿主：Rabbit
反应种属：Human;Mouse;Rat
克隆性：见优宁维官网
产品描述：
IL-10 is an anti-inflammatory cytokine that is produced by T cells, NK cells and macrophages (1,2). IL-10 initiates signal transduction by binding to a cell surface receptor complex consisting of IL-10 RI and IL-10 RII (1). Binding of IL-10 leads to the activation of Jak1 and Tyk2, which phosphorylates Stat-3 (1,3). The anti-inflammatory activity of IL-10 is due to its ability to block signaling through other cytokine receptors, notably IFNγ receptor, by upregulating expression of SOCS-1 (1,3). In addition, IL-10 promotes T cell tolerance by inhibiting tyrosine phosphorylation of CD28 (4,5). IL-10 is an important negative regulator of the immune response, which allows for maintenance of pregnancy (1). In contrast, increased IL-10 levels contribute to persistent Leishmania major infections (6).
爱必信品牌介绍：
更多详情请了解优宁维网站》》
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文献和实验. A rabbit polyclonal antisera is also available (101c500; BAbCO) but in our hands this was less reactive. The antibody cleanup protocol is now a seperate document Protocol Cells are grown in 5 mls of YPAD to an O.D. 600 of 0.75 to 1.
suspended in PBS. A rabbit polyclonal antisera is also available (101c500; BAbCO) but in our hands this was less reactive. The antibody cleanup protocol is now a seperate document Protocol Cells are grown in 5 mls of YPAD to an O.D. 600
Protocol for intracytoplasmic staining of cytokines for FACS analysis
; Pharmingen 18195A) for FL2 anti-IL10 (JES5.16E3-PE; Pharmingen 18435A) for FL1 anti-TGF-beta (rabbit anti pan TGF-beta; R&D Systems AB-100-NA home made FITC conjugate) for FL2 8) Wash extensively in saponin buffer followed by PBS + 0.1% azide
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