- 询价
- Absin
- abs136474
- 上海
- 2025年08月26日
- Rabbit
- 见优宁维官网
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
Rabbit anti-Histone H4R3me2a Polyclonal mAb
- 抗体英文名：
Rabbit anti-Histone H4R3me2a Polyclonal Antibody
- 靶点：
见优宁维官网
- 浓度：
1mg/ml
- 宿主：
Rabbit
- 适应物种：
见优宁维官网
- 保质期：
见优宁维官网
- 抗原来源：
见优宁维官网
- 级别：
优级
- 库存：
999
- 供应商：
Absin
- 标记物：
见优宁维官网
- 克隆性：
见优宁维官网
- 保存条件：
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
- 免疫原：
A synthetic methylated peptide derived from human Di-Methyl-Histone H4 around the methylation site of Arg3.
- 规格：
100ug///50ug
别名：兔抗组蛋白H4R3me2a多克隆抗体A synthetic methylated peptide derived from human Di-Methyl-Histone H4 around the methylation site of Arg3.WB: 1:500-1:2000, IP: 1:50-1:100, IF: 1:50-1:200, ELISA: 1:20000-1:40000, CHIP: 1:50-1:200Store at -20 °C for one year. Avoid repeated freeze/thaw cyclesChIP,Elisa,FlowCytometry,ICC,ICC-IF,IF,IP,WB,other见优宁维官网RabbitHuman;Mouse;Rat
克隆性：见优宁维官网
产品描述：
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element.
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文献和实验. A rabbit polyclonal antisera is also available (101c500; BAbCO) but in our hands this was less reactive. The antibody cleanup protocol is now a seperate document Protocol Cells are grown in 5 mls of YPAD to an O.D. 600 of 0.75 to 1.
suspended in PBS. A rabbit polyclonal antisera is also available (101c500; BAbCO) but in our hands this was less reactive. The antibody cleanup protocol is now a seperate document Protocol Cells are grown in 5 mls of YPAD to an O.D. 600
," made up of fragments of up to seven nucleosomes in length, is incubated with an antiserum directed against the histone modification of interest. The antibody-bound fraction is separated from the unbound fraction and, after extraction of genomic DNA from the bound
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