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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
Rabbit anti-SGK1 Polyclonal mAb
- 抗体英文名:
Rabbit anti-SGK1 Polyclonal Antibody
- 靶点:
见优宁维官网
- 浓度:
1mg/ml
- 宿主:
Rabbit
- 适应物种:
见优宁维官网
- 保质期:
见优宁维官网
- 抗原来源:
见优宁维官网
- 级别:
优级
- 库存:
999
- 供应商:
Absin
- 标记物:
见优宁维官网
- 克隆性:
见优宁维官网
- 保存条件:
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
- 免疫原:
A synthesized peptide derived from Human SGK1(Accession O00141), corresponding to amino acid residues G242-L264.
- 规格:
100ug///50ug
别名:兔抗SGK1多克隆抗体A synthesized peptide derived from Human SGK1(Accession O00141), corresponding to amino acid residues G242-L264. WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000Store at -20 °C for one year. Avoid repeated freeze/thaw cyclesWB,IHC,ELISA见优宁维官网RabbitHuman;Mouse;Rat
克隆性:见优宁维官网
产品描述:
Serum and glucocorticoid-inducible kinase (SGK) is a serine/threonine kinase closely related to Akt (1). SGK is rapidly induced in response to a variety of stimuli, including serum, glucocorticoid, follicle stimulating hormone, osmotic shock, and mineralocorticoids. SGK activation can be accomplished via HGF PI3K-dependent pathways and by integrin-mediated PI3K-independent pathways (2,3). Induction and activation of SGK has been implicated in activating the modulation of anti-apoptotic and cell cycle regulation (4-6). SGK also plays an important role in activating certain potassium, sodium, and chloride channels, suggesting its involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion (2). SGK is negatively regulated by ubiquitination and proteasome degradation (7). The MAP kinase family member BMK1 interacts with and activates SGK by phosphorylation at serine 78 (6).
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更多详情请了解优宁维网站》》
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文献和实验Immunofluorescence: Rabbit Anti-Murine RELMα
for 25 minutes. 3. Incubate the tissue section with blocking buffer for 20 minutes. 4. Incubate the tissue section overnight at 4?C with Rabbit Anti-Murine RELMα at 4.0 ng/mL in 1X PBS with 0.01% Triton-X and 0.5% BSA. Wash the slide
Polyclonal Antibody Production
Zealand White Rabbits and have them delivered to the ARC. Don’t put all your eggs in one basket only using one rabbit. Let them get accustomed to there home there for two weeks before the first pre-bleed. Pre-bleed
. A rabbit polyclonal antisera is also available (101c500; BAbCO) but in our hands this was less reactive. The antibody cleanup protocol is now a seperate document Protocol Cells are grown in 5 mls of YPAD to an O.D. 600 of 0.75 to 1.
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