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- 文献和实验
- 技术资料
- 抗体名:
SARS N (nucleocapsid)/HRP单克隆抗体
- 抗体英文名:
Anti-SARS N (nucleocapsid)/HRP Monoclonal Antibody
- 靶点:
SARS N (nucleocapsid)
- 浓度:
WB 1:500-2000.
- 应用范围:
WB
- 宿主:
Mouse
- 保质期:
Store at -20℃. Avoid freeze / thaw cycles.
- 抗原来源:
Recombinant protein of SARS N (nucleocapsid)
- 级别:
实验级
- 库存:
999
- 供应商:
北京索莱宝科技有限公司
- 标记物:
Biotin,Cy3,Cy3.5,Cy5,Cy5.5,Cy7,FITC,HRP,RBITC,SAlexa Fluor 350,SAlexa Fluor 405,SAlexa Fluor 488,SAlexa Fluor 532,SAlexa Fluor 555,SAlexa Fluor 568,SAlexa Fluor 594,SAlexa Fluor 633,SAlexa Fluor 640,SAlexa Fluor 647,SAlexa Fluor 660,SAlexa Fluor 680,SAlexa Fluor 750
- 克隆性:
Monoclonal Antibody
- 保存条件:
Store at -20℃. Avoid freeze / thaw cycles.
- 形态:
干粉/液体
- 亚型:
IgG2b
- 免疫原:
Recombinant protein of SARS N (nucleocapsid)
- 规格:
100ul
【抗体靶点简介】
The nucleocapsid (N) protein of SARS-coronavirus (SARS-CoV) is the key protein for the formation of the helical nucleocapsid during virion assembly. The nucleocapsid (N) protein of SARS-CoV enters the host cell together with the viral RNA and interferes with several cellular processes. Some of these processes involve interactions between SARS-CoV N protein and host-cell proteins. It has also been demonstrated that the SARS-CoV N protein can bind to DNA in vitro. These interactions might have a role in the pathology of SARS. The N protein may be of potential value in vaccine development for specific prophylaxis and treatment against SARS.【实验操作视频参考】
![]() Western blot实验参考操作流程 | ![]() Immunohistochemistry实验参考操作流程 |
抗体在科研领域应用十分广泛,对解析生物学过程、探究疾病发病机制、创新药物研发与临床治疗研究均起到关键支撑作用。
索莱宝现有30000余种经过严格实验验证的抗体产品,全面覆盖神经科学、细胞凋亡、表观遗传学等热门研究方向,适配 WB、IHC、ELISA、IP、co-IP 等主流实验技术场景。包括内参标签抗体,以及多种标记类型的一抗、二抗,充分满足客户多样化实验选型需求。索莱宝拥有成熟专业的抗体制备与质量检测平台,从免疫制备、纯化提纯到实验验证全流程严格筛选评估,全方位保障每一支抗体的性能与品质。
此外,索莱宝已完善一站式技术服务平台,可提供 WB、IHC、IF、IP、流式检测、细胞实验等常规检测,以及多克隆/单克隆抗体定制等个性化定制服务,为科研用户提供全方位解决方案。
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文献和实验Establishment and Characterization of Monoclonal Antibodies Against SARS Coronavirus
the spike protein (S) and the nucleocapsid protein (N), enabling the immunological detection of SARS-CoV by immunofluorescence staining, immunoblot, or an antigen-capture ELISA system. In addition, several S protein-specific antibodies are shown
Monoclonal Antibody Production Protocol
buffer. (ex: HRP is 2000X). Leave 1 hr at room temp or O.N at 4°C. SUBSTRATE Dissolve substrate in water. Wash plate 4 times with PBS + 0.1% Tween 20 (use TBS instead of PBS for AP). Add 100 ul of substrate to every well. Match color development
Protocol for anti-HA antibody Western Blotting
of interest. 2) Transfer proteins in acrylamide gel to nylon membrane. 3) Block overnight @4℃ in PBS (or TBS, it doesn't matter) with 5% non-fat dry milk. I dilute 2.5 mg in 50 ml. 4) Wash off excess milk sol'n with PBS x3 .(important if you are re-using your antibody
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