- ￥4300
- Solarbio|ActivAb
- 北京
- K009376M
- 2025年08月20日
- WB
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein of SARS N (nucleocapsid)
- 亚型：
IgG2b
- 保存条件：
Store at -20℃. Avoid freeze / thaw cycles.
- 克隆性：
Monoclonal Antibody
- 标记物：
Biotin，Cy3，Cy3.5，Cy5，Cy5.5，Cy7，FITC，HRP，RBITC，Solar Fluor 350，Solar Fluor 405，Solar Fluor 488，Solar Fluor 532，Solar Fluor 555，Solar Fluor 568，Solar Fluor 594，Solar Fluor 633，Solar Fluor 640，Solar Fluor 647，Solar Fluor 660，Solar Fluor 680，Solar Fluor 750
- 抗原来源：
Recombinant protein of SARS N (nucleocapsid)
- 级别：
实验级别
- 库存：
现询
- 供应商：
北京索莱宝科技有限公司
- 宿主：
Mouse
- 应用范围：
WB
- 浓度：
WB 1:500-2000.
- 靶点：
SARS N (nucleocapsid)
- 抗体英文名：
Anti-SARS N (nucleocapsid)/HRP Monoclonal Antibody
- 抗体名：
N [SARS coronavirus ShanghaiQXC2]
- 规格：
100ul
| 基本信息 | |
| 英文名称 | Anti-SARS N (nucleocapsid)/HRP Monoclonal Antibody |
| 宿主 | Mouse |
| 克隆类型 | Monoclonal Antibody |
| 亚型 | IgG2b |
| 克隆号 | 8G8A |
| 预测交叉反应 | SARS |
| 应用 | WB |
| 稀释比例 | WB 1:500-2000. |
| 密度 | |
| 靶标 | SARS N (nucleocapsid) |
| 别名 | N [SARS coronavirus ShanghaiQXC2] |
| 背景说明 | The nucleocapsid (N) protein of SARS-coronavirus (SARS-CoV) is the key protein for the formation of the helical nucleocapsid during virion assembly. The nucleocapsid (N) protein of SARS-CoV enters the host cell together with the viral RNA and interferes with several cellular processes. Some of these processes involve interactions between SARS-CoV N protein and host-cell proteins. It has also been demonstrated that the SARS-CoV N protein can bind to DNA in vitro. These interactions might have a role in the pathology of SARS. The N protein may be of potential value in vaccine development for specific prophylaxis and treatment against SARS. |
| 免疫原 | Recombinant protein of SARS N (nucleocapsid) |
| Public Immunogen Range | 1-422/422aa |
| 理论分子量 | 46kDa |
| EC | |
| 纯化方法 | Affinity purification |
| 储存液 | 0.01M TBS(pH7.4) with 1% BSA，0.03% Proclin300 and 50% Glycerol. |
| 储存条件 | Store at -20℃. Avoid freeze / thaw cycles. |
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文献和实验Establishment and Characterization of Monoclonal Antibodies Against SARS Coronavirus
the spike protein (S) and the nucleocapsid protein (N), enabling the immunological detection of SARS-CoV by immunofluorescence staining, immunoblot, or an antigen-capture ELISA system. In addition, several S protein-specific antibodies are shown
Monoclonal Antibody Production Protocol
buffer. (ex: HRP is 2000X). Leave 1 hr at room temp or O.N at 4°C. SUBSTRATE Dissolve substrate in water. Wash plate 4 times with PBS + 0.1% Tween 20 (use TBS instead of PBS for AP). Add 100 ul of substrate to every well. Match color development
Protocol for anti-HA antibody Western Blotting
of interest. 2) Transfer proteins in acrylamide gel to nylon membrane. 3) Block overnight @4℃ in PBS (or TBS, it doesn't matter) with 5% non-fat dry milk. I dilute 2.5 mg in 50 ml. 4) Wash off excess milk sol'n with PBS x3 .(important if you are re-using your antibody
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