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2-8°C
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文献和实验here will result in expression of fluorescent proteins in cell bodies, dendrites, and axons in targeted neurons, and can be easily adapted to address various experimental questions. Curr. Protoc. Neurosci. 59:1.20.1?1.20.18. © 2012 by John Wiley & Sons
Pollen Development in Anthers of Arabidopsis
to the cover slip to squash preps for viewing. 14. View and photograph the slides. Use a green filter for enhanced contrast and Ilford FP4 film. Within the buds obtained from an inflorescence you should see pre-meiotic pollen mother cells, meiosis
Preparation of probes for in situ hybridization
. The polylinker often contains GC rich stretches that induce cross-hybridization with ribosomal RNA (Witkiewicz H, Bolander ME, Edwards DR, 1993, Biotechniques vol 14, pp 458-463). III. Chemical conjugation of the amino-modified nucleic acids with activated
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