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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
2-8°C
- 保质期:
6个月
- 库存:
100
- 供应商:
百欧泰
- 规格:
10mg/ml
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文献和实验7.8 or Binding Buffer.(6) BD tubing 14-170-12F (Fisher).(7) Polyethylene tubing Clay Adanes.(8) 18 Gauge needles.(9) Binding Buffer (BioRad): make 1L. 314g/L ddH2O and filter through 0.22 um, filter. pH=9.0.(10) Elution buffer (BioRad): make 500 ml. 11g/500 ml
How to perform automated counts of fluorescently stained cells.
propidium iodide / mL PBS) Hemocytometer U.V. fluorescent microscope with camera Computer with ImageJ and image cropping software. Procedure Fix Cells by diluting them 50% in EtOH. 1. To an eppendorf add 0.1 mL cells in PBS or media 2.
cat. #1530-6160 ($161.00) 50 mM Tris pH7.8 40 g ammonium sulfate for every 100 ml Sup. 4L 100 mM Tris/pH7.8 or Binding Buffer BD tubing 14-170-12F (Fisher) Polyethylene tubing Clay Adanes. 18 Gauge
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