(Methyl3,4,6-Tri-O-acetyl-2-de

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duplex by 2'-deoxy or 2'-O-methyl oligoribonucleotides abolished silencing in fly extract (Elbashir et al. 2001c). In mammalian cells, however it seems possible to substitute the sense siRNA by a 2'-O-methyl oligoribonucleotide (Ge et al. 2003)Useful

Oligodeoxynucleotide Containing S‐Functionalized 2′‐Deoxy‐6‐Thioguanosine: Facile Tools for Base‐Selective and Site‐Specific Internal Modification of

oligonucleotides with enzyme, photochemistry, or catalytic DNA. The functionality?transfer ODN (FT?ODN), which contains 2??deoxy?6?thioguanosine (6?thio?dG) functionalized with the 2?methyliden?1,3?diketone group, is hybridized with the target RNA to trigger

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of the antisense strand. All other hairpin RNAs behaved very similar to siRNA duplexes. Substitution of one or both strands of a siRNA duplex by 2''-deoxy or 2''-O-methyl oligoribonucleotides abolished silencing in fly extract (Elbashir et al. 2001c