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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
(1S, 4S)-2,5-Diaza-bicyclo2.2.1heptane dihydrochloride
- 库存:
999
- 供应商:
智溯科ZSK
- CAS号:
5260-20-8
- 规格:
10mg///100mg///200mg
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文献和实验BACs, PACs, P1s and Plasmid Isolation Protocol (standard protocol)
3 and gently mix by inverting 15-20 times until a flocculent white precipitate forms. Incubate on ice for 5 minutes. Do not vortex, as this will result in shearing. 6. Centrifuge at $ 13,000 x g for 10 minutes at 4/C. Promptly proceed to the next step
off the EtOH and let pellet air dry (or speed vac dry on low heat). 14. Resuspend in 15-20 ul RNase-free water. 15. Spin down for 30 seconds and quantitate a 1:100 dilution at 260nm. Note: Typically yields should be no greater than 2% of the total RNA input
E.Z.N.A. Endo-Free BACs, PACs, P1s Protocol
when not in use). 5. Add 200 ul of Chilled Buffer T3 and gently mix by inverting 15-20 times until a flocculent white precipitate forms. Incubate on ice for 5 minutes. Do not vortex, as this will result in shearing. 6. Centrifuge at $ 13,000 x g for 10
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