- ￥2367.78
- BioLegend
- 301028
- 美国
- 2025年08月18日
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
Alexa Fluor® 700 anti-human CD8a
- 抗体英文名：
Alexa Fluor® 700 anti-human CD8a
- 供应商：
上海善然生物科技有限公司
- 保存条件：
2-8℃
- 规格：
100ug
Alexa Fluor® 700 anti-human CD8a Antibody
RPA-T8
Catalog# / Size
301028 / 100 µg
Clone
Regulatory Status RUO
Workshop IV T171
Other Names T8, Leu2
Isotype Mouse IgG1, κ
Description CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer
(CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin
superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK
cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with
MHC class I-restricted T cell receptors in antigen recognition and T cell activation, and has
been shown to play a role in thymic differentiation. Two domains in CD8a are important for
function: the extracellular IgSF domain binds the α domain of MHC class I and the
cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.
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文献和实验Vybrant® DyeCycle Violet Stain
Fluor® 488 and PE channels Eliminate or correct for cell aggregates during data analysis using gating or modeling software Human and rodent stem cells efflux Vybrant® DyeCycle™ Violet stain, and this is the basis for the Side Population
Immune Response Biomarker Profiling
实验试剂 ProtoArray® Human Protein Microarray v5.0 Blocking Buffer and Washing Buffer 10 × Synthetic Block [ 详细信息： Invitrogen ] Alexa Fluor® 647 Goat Anti-Human IgG [ 详细信息： Invitrogen ]
细胞仪检测。或者继续 E1 步 DNA 染色。 E. DNA 染色（可选） 1. 将细胞重悬在 0.5 ml DNA 染色液中（例如 DRAQ5® #4084）。 2. 室温至少孵育 5 分钟。 3. 流式细胞仪进行分析。 ☀ 推荐的二抗： 抗兔： Anti-Rabbit IgG（H+L）， F（ab'）2 Fragment（Alexa Fluor® 488 Conjugate）#4412 Anti-Rabbit IgG（H+L）， F（ab
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