APC anti-human CD16 Recombina

Catalog# / Size：C-0018-41 /25Tests/C-0018-42 /100Tests

Clone：KEL-CD16-001

Regulatory Status：RUO

Other Names：FcγRIII, IGFR3, FCG3, FCGR3, FCGRIII, Fc gamma receptor, Fc gamma receptor 3, CD16a

Isotype：human IgG1, κ

Description：CD16 is known as low affinity IgG receptor III (FcγRIII). It is expressed as two distinct forms (CD16a and CD16b). CD16a (FcγRIIIA) is a 50-65 kD polypeptide-anchored transmembrane protein. It is expressed on the surface of NK cells, activated monocytes, macrophages, a subset of T cells and placental trophoblasts in humans. CD16b (FcγRIIIB) is a 48 kD glycosylphosphatidylinositol (GPI)-anchored protein. Its extracellular domain is over 95% homologous to that of CD16a, and it is expressed specifically on neutrophils. CD16 binds aggregated IgG or IgG- antigen complex which functions in NK cell activation, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC).

Verified Reactivity：Human

Antibody Type：Monoclonal

Host Species：Mouse

Immunogen：NK cell-enriched fraction from human peripheral blood.

Formulation：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation：The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.

Concentration：Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)

Storage & Handling：The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application：FC - Quality tested

Recommended Usage：Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser：Red Laser (633 nm)

Application Notes：The epitope recognized by clone KEL-CD16-001 is in the first membrane distal Ig-like domain of the CD16 molecule

                                 Donor variability has been observed for clone KEL-CD16-001 staining1 , especially on granulocytes