- ￥150.48
- AMEKO
- 中国
- AB65502-100g
- 2025年08月14日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
RT
- 库存：
99
- 供应商：
联硕生物
- CAS号：
86088-90-6
- 规格：
100g
纯度：BR
保存温度：RT
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文献和实验Isolation of Total RNA from Difficult Tissues
challenges for total RNA isolation. Some of the demanding tissues requiring more manipulation and fine-tuning during the RNA isolation procedure are heart, brain, thymus and spleen. Here we address problems we have encountered, and offer troubleshooting
Modified Total RNA Protocol for Heart, Muscle, Brain and Skin tissue
实验步骤 Note: Always use less starting material ( 1. Disrupt the tissue and homogenize lysate in 6.0mL TRK lysis buffer with a rotorstator homogenizer. Transfer the homogenate to a 50ml centrifuge tube which is capable
Preparation of nuclear extract and cytoplasmic extract
PMSFBuffer D (Dialysis Buffer): For 2 L20 mM HEPES,pH 7.9 1 M HEPES 7.9 40 ml20 % Glycerol Glycerol 400 ml100 mM KCl3 M KCl66.67 ml0.2 mM EDTA 0.5 M EDTA 800 µl0.5 mM DTT 1M DTT 1 ml0.2 mM PMSF 0.2 M PMSF 2 mlSplicing Extract Preparation1.Resuspend cell
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