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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
2-8°C
- 英文名:
(S)-O-Desmethyl Naproxen
- 库存:
99
- 供应商:
联硕生物
- CAS号:
52079-10-4
- 规格:
5mg
纯度:≥90%
保存温度:2-8°C
英文名称:(S)-O-Desmethyl Naproxen
分子式:C13H12O3
ZJRBLDYXPGMTUFQNVWAIESCHKO
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文献和实验the pellet. Add 400 ul 75% EtOH, vortex briefly. Spin at 4°C, 4000 rpm for 10 min. Remove s/n with pipet and invert racked tubes in hood with door half shut. Approx. 10-15 minutes; do not overdry. Add 300 l DEPC-treated ddH2 O
DNA EXTRACTION FROM MICRODISSECTED PARAFFIN SECTIONS
(dip 3-6 times or leave ~15-60 seconds). The section should not be too dark. The methyl green concentration can be varied if sections are generally too dark or too light. It's better to start with a lower concentration. dH2O 1-5 mins
CHROMATIN IMMUNOPRECIPITATION (CHIP) PROTOCOL FOR YEAST
down in Sorvall--5 K, 5 minutes, 4℃. Drain. Resuspend cells in 5 mL HEPES/sorb to wash. Spin down (5 K, S minutes, 4℃). Drain. Resuspend in 5 mL HEPES/sorb with 0.5 mM PMSF. Add 30-40 mL 1 mg/mL oxalyticase (can also use 2 mg zymolyase here, as preferred
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