- ￥1710
- AMEKO
- 中国
- AB62782-5mg
- 2025年08月14日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
2-8℃
- 库存：
99
- 供应商：
联硕生物
- CAS号：
1351975-56-8
- 规格：
5mg
纯度：HPLC≥98%
保存温度：2-8℃
分子式：C20H28O9
ILXKMEWVNPCBSTUHROQZFJDYGA
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文献和实验CHROMATIN IMMUNOPRECIPITATION (CHIP) PROTOCOL FOR YEAST
down in Sorvall--5 K, 5 minutes, 4℃. Drain. Resuspend cells in 5 mL HEPES/sorb to wash. Spin down (5 K, S minutes, 4℃). Drain. Resuspend in 5 mL HEPES/sorb with 0.5 mM PMSF. Add 30-40 mL 1 mg/mL oxalyticase (can also use 2 mg zymolyase here, as preferred
Single and Double FISH protocols for Drosophila
7. 1.5 ml microcentrifuge tubes, autoclaved. 1.3 Post- Fixation and Hybridization of Whole-mount Embryos 1. PBT solution: 1X PBS plus 0.1% Tween-20. 2. 40% formaldehyde solution, prepared that day). 3. 20mg/ml Proteinase K (Sigma
RNA in situ protocol for DNA and RNA probes
for 3-5 min. with 50 ug/ml proteinase K in PBT**. **When using riboprobes, it is necessary to reduce or eliminate the proteinase K treatment. We use 4 ug/ml proteinase K for 3 min. 11) Stop the protease by washing 2x with PBT containing 2 mg/ml
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