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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
2-8℃
- 英文名:
12alpha-HYDROXYROTENONE
- 库存:
99
- 供应商:
联硕生物
- CAS号:
509-96-6
- 规格:
5mg
纯度:HPLC≥98%
保存温度:2-8℃
英文名称:12alpha-HYDROXYROTENONE
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文献和实验ELISA (Enzyme-Linked ImmunoadSorbent Assay)
and PSAR (Peroxidase-conjugated Swine Anti Rabbit) for Ab raised in rabbits. 10) Wash 4x with 1x PBS containing 0.01% tween. 11) Add substrate (100ul/well). 12) Incubate for 1-5 mins in the dark at RT. Check reaction for colour change. Wells
(交流)ELISA (Enzyme-Linked ImmunoadSorbent Assay)
in rabbits.10) Wash 4x with 1x PBS containing 0.01% tween.11) Add substrate (100ul/well).12) Incubate for 1-5 mins in the dark at RT. Check reaction for colour change. Wells should turn blue.13) Stop reaction by the addition of 25ul 0.1M H2SO4/well.14) Read
T-cell dependent antigen specific in situ staining (Pe)
) Levamisole 1mM (12 mg/50 ml) (Sigma L9756) 20 mg Naphtol As BI phosphate (stock solution 40 mg/ml in NN-DM formamide, anhydrous, kept at -20°C) (Sigma N2250, the cheapo) 10 mg Fast Blue BB Diazonium salt (Sigma F3378) Shake
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