- ￥44.46
- AMEKO
- 中国
- AB67857-100mg
- 2025年08月14日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
2-8℃
- 英文名：
DavePhos-Pd-G3
- 库存：
99
- 供应商：
联硕生物
- CAS号：
1445085-87-9
- 规格：
100mg
纯度：98%
保存温度：2-8℃
英文名称：DavePhos-Pd-G3
分子式：C39H49N2O3PPdS
PUAJYNCOEXDQTRSVBWGZMILHFK
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文献和实验Phycoerythrin conjugation protocol
30 µl DTT (0.5M, i.e. 77 mg/ml in PBS). Incubate for 30 mins at room temperature. Separate derivatized PE and Ab from activators on G-10 columns. You should now have approximately 2 ml of each reactant. Mix derivatized
Conjugation of Antibody to HRP
the first peak. Preparation of Ab:HRP Conjugate Immediately mix the HRP with the thiolated antibody at 1 mg HRP per mg antibody. Gently stir for 16-20 hours at 2-8oC. Dialyze against 3 changed of PBS, changing every 2 hours, 100X the conjugate
Whole mount in situ hybridization with digoxygenin probes
Whole mount in situ hybridization with digoxygenin probes 1. Probe labelling (DNA) DNA template (100 ng - 1 µg ) in dH20 5 µl pd(N)6 (10 mg/ml) 10 µl Mix template and hexamer, boil 5 min, chill on ice 3 min then add
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