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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 英文名:
N-STEAROYL-D-SPHINGOMYELIN
- 库存:
99
- 供应商:
联硕生物
- CAS号:
58909-84-5
- 规格:
1mg
纯度:>99%
保存温度:-20℃
英文名称:N-STEAROYL-D-SPHINGOMYELIN
分子式:C41H83N2O6P
WVFQPEDLZYXKJIHCASRONUBMGT
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文献和实验Yeast Chromatin Immunoprecipitation (ChIP)
ice, and store at �70 degrees C Day 5 1. Thaw extracts on ice and transfer a volume of extract (1mg total protein) to a new 1.5 ml tube for IP 2. Include necessary controls (i.e. no Ab control; untagged strain + Ab) 3. Bring all 1mg aliquots
Yeast Chromatin Immunoprecipitation (ChIP)
total protein)to a new 1.5 ml tube for IP 2.Include necessary controls (i.e.no Ab control; untagged strain+ Ab) 3.Bring all 1mg aliquots to 200μl with ChIP lysis buffer+protease inhibitors,mix well (thorough mixing is very important) 4.Transfer 2μl
Single and Double FISH protocols for Drosophila
of IgG (H+L) 1/2000 dilution of a 1 mg/ml stock solution; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA; Cat No. 115-226-062]. 7. Donkey anti-sheep antibody conjugated to CY3 [F(ab')2 fragment of IgG (H+L); 1/2000 dilution of a 1mg/ml
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