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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
2-8℃
- 库存:
99
- 供应商:
联硕生物
- CAS号:
13632-08-1
- 规格:
100mg
纯度:97%
保存温度:2-8℃
分子式:C10H12ClNO3S
LHQBXKURNEYTFAWCIOPDSMVJGZ
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文献和实验total of 32 ml of 100:10 TE buffer, 5 ml of 5 mg/ml ethidium bromide, and 37 g cesium chloride (Var Lac Oid Chemical Co., Inc.) (final concentration of cesium chloride should be 1 g/ml). 10a. Transfer the sample into 35 ml polyallomer centrifuge
Phycoerythrin conjugation protocol
30 µl DTT (0.5M, i.e. 77 mg/ml in PBS). Incubate for 30 mins at room temperature. Separate derivatized PE and Ab from activators on G-10 columns. You should now have approximately 2 ml of each reactant. Mix derivatized
is an indirect expression of the monoclonal antibody amount in the sample.Methods1 Obtain a 96-well plate and rinse 3x with PBS.2 Add 50 µl of a 1 mg/ml of antigen (venom, serum, or fraction), incubate for 30 min at 37°C.3 Rinse with PBS 3x.4 Add 5% powdered milk
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