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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
2-8℃
- 库存:
99
- 供应商:
联硕生物
- 规格:
100ul
保存温度:2-8℃
UVXTJCANSFEBYIPLGRHDQKWZOM
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文献和实验James Hardwick's angiotensin assay protocol
that you use for precipitation or you will risk losses. 1.Immunoprecipitate the protein from 3 x106 cells using 45 microliters S.aureus bugs.Resuspend the immunoprecipitates in 12 microliters kinase buffer (40 mM PIPES,pH 7.0,10 mM MnCl2)at 4℃. 2.The kinase
Kamps's Western Blotting Protocol
/filter sandwich in the holder such that the proteins will migrate from the gel to the membrane,in the direction of the positive (red)electrode (anode).4)Transfer for 60 min at 60 Volts (or 3600 Volt X Min).Some very small proteins will pass
E-Z 96 X-press Plasmid Vacuum Protocol
collection plate as a support to give the collection plate a proper position. 15. Add 100-150 μl Elution Buffer (10mM Tris-HCl, pH 8.5) or sterile water to each well of the E-Z 96® X-press Plate, let stand for 2 minutes. Apply maximum vacuum for 5-10
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